Isonicotinic Acid

CAS Number 55-22-1
Molecular Formula C6H5NO2
Molecular Weight 123.112 g/mol
InChI Key TWBYWOBDOCUKOW-UHFFFAOYSA-N
LogP 0.32
Synonyms
  • Isonicotinic acid
  • Pyridine-4-carboxylic acid
  • 4-Pyridinecarboxylic acid
  • 55-22-1
  • 4-Pyridinecarboxylic acid
  • 4-Carboxypyridine
  • 4-Picolinic acid
  • 4-Pyridylcarboxylic acid
  • Acide isonicotinique
  • acido isonicotinico
  • Isonicotinsaure
  • NSC 1483
  • p-Pyridinecarboxylic acid
  • γ-Picolinic acid
  • γ-Pyridinecarboxylic acid
  • 1,4-Dihydroisonicotinic acid
  • EINECS 200-228-2
  • Acide iso-nicotinique
  • UNII-Y8SYN761TQ
  • gamma-picolinic acid
  • gamma-pyridinecarboxylic acid
  • pyridine-4-carboxylic acid

Applications:


HPLC Separation of Pyridinecarboxylic Acids


Pyridinecarboxylic acids exist as three isomers with different position of carboxylic acid relative to nitrogen in pyridine. Three isomers of pyridinecarboxylic acid (picolinic or 2-pyridinecarvoxylic acid, niacin or 3-pyridinecarboxylic acid, isonicotinic or 4-pyridinecarboxylic acid), along with pyridinedicarboxylic acid, are separated on a Primesep 100 column. Pyridinecarboxylic acids have a similar empirical formula, and are very similar in terms of hydrophobicity and ionic properties. Small differences in these properties are enough to achieve good separation on cation-exchange mixed-mode HPLC column like Primesep 100. Retention time for all compounds is controlled by the amount of acetonitrile and amount of ions in the mobile phase. Ions in the mobile phase can be created by organic and inorganic acids and corresponding salt buffers. Various detection techniques can be used for monitoring pyridinecarboxylic acids. Other ionizable isomers can be successfully separated on mixed-mode columns.



Application Analytes:

Isonicotinic Acid
Nicotinic Acid
Organic Acids
Picolinic Acid
Pyridinedicarboxylic Acid

HPLC Separation of Vitamin C, Vitamin Group B, and Related Impurities



Vitamin C (ascorbic acid) and Vitamins Group B are separated on Obelisc N mixed-mode column. Method can be used in quantitation and determination of polar vitamins in various formulations and dietary supplements. HPLC method can be based on UV, Evaporative Light Scattering Detection (ESLD), RI or MS detection. Effect of sample matrix can be eliminated by changing mobile phase conditions. Buffer concentration, buffer pH and amount of ACN will affect every vitamin differently due to difference in polar and ionic properties.



Application Analytes:

3-Aminopyridine
4-Aminopyridine/Fampridine
Ascorbic Acid
Barbituric Acid
Isonicotinic Acid
Nicotinic Acid
Pyridoxine
Vitamin B2 (Riboflavin)
Vitamin B6 (Pyridoxine)