| CAS Number | 16846-24-5 |
|---|---|
| Molecular Formula | C42H69NO15 |
| Molecular Weight | 828.006 g/mol |
| InChI Key | XJSFLOJWULLJQS-HTYUZQQASA-N |
| LogP | 3.06 |
| Synonyms |
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Applications:
Separation of Antibiotics in Mixed-mode chromatography
A complex mixture of sulphonamide, macrolide, tetracycline and fluoroquinolone antibiotics were separated in one run using mixed-mode chromatography with LC/MS -compatible conditions. All compounds are separated based on reversed-phase and/or ion-exchange mechanism. Method can be used for analysis of various classes of antibiotics and related impurities in different sample matrices (blood, urine, soil, waste water).
| Column | Obelisc R, 2.1x150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN - 0-25%, 6 min, 25-70% 14 min |
| Buffer | Gradient Formic Acid - 0.05%-0.3%, 10 min, 14 min hold |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Antibiotic, Drug, Hydrophobic, Ionizable |
| Analyzing Compounds | Sulphanilamide, Sulphaguanidine, Oxytetracycline, Marbofloxacin, Sulphadiazine, Chlortetracycline, Nofloxacin, Spiramycin, Cefalonium, Tylosin, Josamycin, Sulphamethoxazole, Dapsone, Sulphaquinoxalline, Flumequine |
Application Analytes:
Carboxymethylcysteine
Chlortetracycline
Dapsone
Flumequine
Josamycin
Marbofloxacin
Norfloxacin
Oxytetracycline
Spiramycin
Sulfamethoxazole
Sulfonamides
Sulphadiazine
Sulphaguanidine
Sulphanilamide
Sulphaquinoxaline
Tylosin
Analysis of Antibiotic Josamycin and Related Impurities in Mixed-Mode Chromatography
| Column | Obelisc R, 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN - 5-30%, 10 min, 5 min hold |
| Buffer | Gradient Formic Acid - 0.05%-0.3%, 10 min, 5 min hold |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
Josamycin is a macrolide antibiotic effective against gram-positive bacteria. It is highly polar and does not retain well on reversed-phase columns. The Mixed-mode column Obelisc R was used to retain josamycin and its related impurities. Primesep D was also used to analyze josamycin impurities because Primesep D does not retain high-molecular weight plasma components. This method is compatible with LC/MS, prep chromatography and can be used for analysis of josamycin in biofluids.
| Column | Primesep D, 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN - 5-50%, 10 min, 3 min hold |
| Buffer | H2SO4 - 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Antibiotic, Drug, Hydrophobic, Ionizable |
| Analyzing Compounds | Josamycin |
Application Analytes:
Josamycin
HPLC Separation of Antibiotics of Group in Mixed-Mode Chromatography
Separation type: Liquid Chromatography Mixed-mode
Primesep D was used to separate the antibiotics marbofloxacin, norfloxacin, josamycin, and flumequine. Primesep D is a reverse-phase column with embedded basic ion-pairing groups designed for analysis of plasma. This method is compatible with LC/MS and prep chromatography.
| Column | Primesep D, 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN - 5-50%, 10 min, 3 min hold |
| Buffer | H2SO4 - 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Antibiotic, Drug, Hydrophobic, Ionizable |
| Analyzing Compounds | Josamycin, Norfloxacin, Marbofloxacin, Flumequine |
Application Analytes:
Flumequine
Josamycin
Marbofloxacin
Norfloxacin