Malic Acid

CAS Number 6915-15-7
Molecular Formula C4H6O5
Molecular Weight 134.088 g/mol
InChI Key BJEPYKJPYRNKOW-UHFFFAOYSA-N
LogP -1.26
Synonyms
  • Malic acid
  • 2-Hydroxybutanedioic acid
  • Butanedioic acid, 2-hydroxy-
  • 6915-15-7
  • 2-Hydroxy-succinic acid
  • 2-Hydroxybutanedioic acid
  • Butanedioic acid, hydroxy-
  • (.+-.)-Malic acid
  • 2-Hydroxyethane-1,2-dicarboxylic acid
  • 2-Hydroxysuccinic acid
  • Acide malique
  • acido malico
  • APFELSAEURE
  • Apfelsaure
  • Butanedioic acid, 2-hydroxy-
  • BUTANEDIOIC ACID, HYDROXY-, (D,L)-
  • Deoxytetraric acid
  • DL-Malic acid
  • DL-MALIC ACID, HYDROXYSUCCINIC ACID
  • Hydroxybutanedioic acid
  • Hydroxysuccinic acid
  • MALIC ACID, DL
  • MALIC ACID, DL-
  • Musashi-no-Ringosan
  • NSC 25941
  • Pomalus Acid
  • R,S(.+-.)-Malic acid
  • α-Hydroxysuccinic acid
  • Caswell No. 537
  • EINECS 210-514-9
  • EINECS 230-022-8
  • EPA Pesticide Chemical Code 051101
  • FEMA No. 2655
  • FEMA Number 2655
  • Kyselina jablecna
  • Succinic acid, hydroxy-
  • R,S(+-)-Malic acid
  • Monohydroxybernsteinsaeure
  • Hydroxybutandisaeure
  • Kyselina hydroxybutandiova
  • UNII-817L1N4CKP
  • Malicum acidum
  • Aepfelsaeure
  • E296
  • H2mal
  • alpha-hydroxysuccinic acid
  • apple acid
  • hydroxybutanedioic acid
  • 41308-42-3
  • 617-48-1
  • 623158-98-5
  • 879715-44-3
  • 1334703-48-8

Applications:


Separation of Diacid: Ion Exclusion mode



Primesep 100 separates a mixture of dicarboxylic acids in ion-exclusion mode with a mobile phase of water, acetonitrile (MeCN, ACN), and sulfuric acid (H2SO4) with UV detection at 210 nm. Baseline resolution of fumaric, maleic, malic, and succinic acids is obtained in less than 8 minutes. The separation combines ion-exclusion and reversed-phase mechanisms in one method.



Application Analytes:

Dicarboxylic Acids
Fumaric Acid
Maleic Acid
Malic Acid
Succinic Acid

Separation of Diacid Hydrophobic and Ion Exclusion Modes



Primesep 200 retains and separates the organic diacids (malic, succinic, fumaric, and maleic) by a combination hydrophobic, reversed-phase interactions and ion exclusion. The separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 210 nm.



Application Analytes:

Dicarboxylic Acids
Fumaric Acid
Maleic Acid
Malic Acid
Succinic Acid

HILIC Separation of Carboxylic Acids



Hydrophilic acids are separated on Obelisc N mixed-mode HILIC column. Seven carboxylic acids are separated based on their polarity and pKa values. Changes in ionization states of acids and stationary phase can be used to control elution order of organic and inorganic acids.



Application Analytes:

Fumaric Acid
Hydroxybenzoic Acid
Malic Acid
Mandelic Acid
Methylmalonic Acid
Organic Acids
Succinic Acid
Tartaric Acid

HPLC Separation of Organic Acids



Application Analytes:

Butanesulfonate
Malic Acid
Organic Acids
Sodium
Succinic Acid

Analysis of Compounds of TCA Cycle on SHARC 1 Column

This is an alternative approach for the separation of the TCA cycle intermediates that was developed using novel hydrogen-bonding HPLC column. The organic nature of the mobile phase helps obtain a highly sensitive LC/MS compatible method.



Application Analytes:

Citric Acid
Fumaric Acid
Isocitric Acid
Maleic Acid
Malic Acid
Succinic Acid

Separation of Compounds in TCA Cycle on Primesep D Column

The citric acid cycle (tricarboxylic acid cycle, Krebs cycle) is a key process in the metabolic pathway by which all aerobic organisms generate energy. Compounds generated during TCA are hydrophilic, acidic compounds. Some of the hydrophilic acids are very strong while others are relatively weaker. All compounds have very low hydrophobicity and do not retain by the reversed-phase mechanism on the C18 or C8 HPLC columns. Citric acid and related products were separated in reversed-phase, anion-exchange mode on the Primesep D mixed-mode column. All hydrophilic, acidic compounds are well separated and demonstrate good shape. This robust method can be used for analysis of components of the TCA cycle and other highly hydrophilic compounds. This method is fully compatible with LC/MS and prep chromatography.



Application Analytes:

Citric Acid
Fumaric Acid
Isocitric Acid
Maleic Acid
Malic Acid
Sodium
Succinic Acid

HPLC Separation of Small Organic Acids on Newcrom B Column
HPLC.cloud View on hplc.cloud  

Condition

Column Newcrom B, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 5/95%
Buffer AmAc pH 5.0, Formic Acid
Flow Rate 1.0 ml/min
Detection CAD (Corona) MS- compatible mobile phase

Description

Class of Compounds Acid, Hydrophilic, Ionizable
Analyzing Compounds Glycolic acid, Succinic Acid, Methylmalonic acid, Malic Acid, Maleic Acid, Citric Acid, Tartaric Acid, Fumaric Acid, Malonic Acid


Application Analytes:

Citric Acid
Fumaric Acid
Glycolic acid sodium salt
Maleic Acid
Malic Acid
Malonic Acid
Methylmalonic Acid
Succinic Acid
Tartaric Acid
dl-Tartaric acid