Complex Mixture of Acids, Bases, Amino Acids, and Neutral Compounds
Primesep 100 separates a mixture of amino acids (tyrosine, phenylalanine), organic acids (benzoic acid, mandelic acid), amines (benzylamine, pyridine), and neutrals (benzonitrile, toluene) in one HPLC run by combining reversed-phase, cation-exchange, and polar interactions. The method is tunable and peak order can be changed significantly by adjusting acetonitrile and trifluoroacetic acid concentrations. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) and compatible with UV, mass spec (LC/MS) and evaporative light scattering (ELSD) detection.
||Primesep 100, 4.6x250 mm, 5 µm, 100A
||MeCN/H2O - 30/70%
||TFA - 0.2
||UV, 210 nm
|Class of Compounds
||Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
||Tyrosine, phenylalanine, Benzoic acid, mandelic acid, Benzylamine, Pyridine, Benzonitrile, Toluene
Amino AcidsBenzoic AcidBenzonitrileBenzylamineMandelic AcidOrganic AcidsPhenylalaninePyridineTolueneTyrosine
HILIC Separation of Carboxylic Acids
Hydrophilic acids are separated on Obelisc N mixed-mode HILIC column. Seven carboxylic acids are separated based on their polarity and pKa values. Changes in ionization states of acids and stationary phase can be used to control elution order of organic and inorganic acids.
Fumaric AcidHydroxybenzoic AcidMalic AcidMandelic AcidMethylmalonic AcidOrganic AcidsSuccinic AcidTartaric Acid