Marbofloxacin

CAS Number 115550-35-1
Molecular Formula C17H19FN4O4
Molecular Weight 362.361 g/mol
InChI Key BPFYOAJNDMUVBL-UHFFFAOYSA-N
LogP -0.328
Synonyms
  • Marbofloxacin
  • 9-Fluoro-3-methyl-10-(4-methylpiperazin-1-yl)-7-oxo-2,3-dihydro-7H-[1,3,4]oxadiazino[6,5,4-ij]quinoline-6-carboxylic acid
  • 7H-1,3,4-Oxadiazino[6,5,4-ij]quinoline-6-carboxylic acid, 9-fluoro-2,3-dihydro-3-methyl-10-(4-methyl-1-piperazinyl)-7-oxo-
  • 115550-35-1
  • Marbofloxacine
  • Marbofloxacino
  • Marbofloxacinum
  • UNII-8X09WU898T
  • Zeniquin

Applications:


Separation of Antibiotics in Mixed-mode chromatography
  A complex mixture of sulphonamide, macrolide, tetracycline and fluoroquinolone antibiotics were separated in one run using mixed-mode chromatography with LC/MS -compatible conditions. All compounds are separated based on reversed-phase and/or ion-exchange mechanism. Method can be used for analysis of various classes of antibiotics and related impurities in different sample matrices (blood, urine, soil, waste water).  

Condition

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 0-25%, 6 min, 25-70% 14 min
Buffer Gradient Formic Acid - 0.05%-0.3%, 10 min, 14 min hold
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds  Antibiotic, Drug, Hydrophobic, Ionizable
Analyzing Compounds Sulphanilamide, Sulphaguanidine, Oxytetracycline, Marbofloxacin, Sulphadiazine, Chlortetracycline, Nofloxacin, Spiramycin, Cefalonium, Tylosin, Josamycin, Sulphamethoxazole, Dapsone, Sulphaquinoxalline, Flumequine
 

Application Analytes:

Carboxymethylcysteine
Chlortetracycline
Dapsone
Flumequine
Josamycin
Marbofloxacin
Norfloxacin
Oxytetracycline
Spiramycin
Sulfamethoxazole
Sulfonamides
Sulphadiazine
Sulphaguanidine
Sulphanilamide
Sulphaquinoxaline
Tetracycline
Tylosin

Analysis of Antibiotic Marbofloxacin and Related Impurities in Mixed-Mode Chromatography
Marbofloxacin is carboxylic acid derivative related to fluoroquinolone antibiotics. Method for analysis of marbofloxacin and related impurities was developed on Obleisc R and Primesep D mixed-mode column. Obelisc R is tri-modal reversed-phase, cation- and anion-exchange column. Primesep D is mixed-mode reversed-phase anion-exchange column.  

Condition 1

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 0-25%, 6 min,  25-70% 14  min
Buffer Gradient Formic Acid - 0.05%-0.3%, 6 min,  14 min hold
Flow Rate 1.0 ml/min
Detection UV, 270 nm

Condition 2

Column Primesep D, 4.6x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 5-50%, 10 min, 3 min hold
Buffer H2SO4 - 0.1%
Flow Rate 1.0 ml/min
Detection UV, 250 nm
 

Description

Class of Compounds  Antibiotic, Drug, Hydrophobic, Ionizable
Analyzing Compounds  Marbofloxacin
 

Application Analytes:

Marbofloxacin

HPLC Separation of Antibiotics of Group in Mixed-Mode Chromatography

Primesep D was used to separate the antibiotics marbofloxacin, norfloxacin, josamycin, and flumequine. Primesep D is a reverse-phase column with embedded basic ion-pairing groups designed for analysis of plasma. This method is compatible with LC/MS and prep chromatography.



Application Analytes:

Flumequine
Josamycin
Marbofloxacin
Norfloxacin