| CAS Number | 94-81-5 |
|---|---|
| Molecular Formula | C11H13ClO3 |
| Molecular Weight | 228.670 g/mol |
| InChI Key | LLWADFLAOKUBDR-UHFFFAOYSA-N |
| LogP | 3.30 |
| Synonyms |
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Applications:
MCPB Analysis Using Obelisc and Primesep Analytical HPLC Columns
MCPB (4-(4-Chloro-methylphenoxy)butanoic acid) is an herbicide used on post-emergence broad-leaf weeds. Its acute toxicity is low to moderate, and has been show to damage liver and kidney tissue. Obelisc R and Primesep 100 were both used to separate MCPB. Primesep 100 is a reverse-phase column with embedded acidic ion-pairing groups while Obelisc R is a mixed-mode column with separates using ionic groups and a long hydrophobic chain. The European Union Reference Laboratory (EURL) included MCPB in an analysis of acidic pesticides using single residue methods. Method is LC/MS compatible and useful for many pesticides.
| Column | Obelisc R, 2.1x150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN - 25-70%, 15 min |
| Buffer | Gradient AmAc pH 3.0- 30-60 mM, 15 min |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Column | Obelisc R, 2.1x150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN - 10-70%, 15 min |
| Buffer | Gradient AmAc pH 3.0- 20-60 mM, 15 min |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Column | Primesep 100, 2.1x150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN - 10-70%, 15 min |
| Buffer | Gradient AmAc pH 3.0- 20-60 mM, 15 min |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | MCPB |
Application Analytes:
MCPB
HPLC Separation of Pesticides, Herbicides, Fungicides and Insecticides on Newcrom BH Column
Herbicides are used to control unwanted plants, they are also known as weedkillers. Insecticides are used to kill insects. Fungicides are used to kill parasitic fungi. Pesticide is a more generic term that includes herbicides, fungicides and insecticides in its definition. All are heavily used in agriculture. By using HPLC, many different pesticides can be separated and their retention characteristics controlled using the Newcrom BH mixed-mode column. It can be used with different organic media such as acetonitrile (ACN) or methanol (MeOH). By varying the concentration of organic modifier and using different buffers like sulfuric acid (H2SO4), ammonium formate (AmFm), or formic acid, separation of desired pesticides can be achieved.
| Column | Newcrom BH, 3.2x100 mm, 5 µm, 100A |
| Mobile Phase | MeCN, MeOH |
| Buffer | H2SO4, Formic acid, AmFm pH 3.0 |
| Flow Rate | 0.6 ml/min |
| Detection | UV 275 nm |
| Class of Compounds | Pesticides, Herbicides, Fungicides, Insecticides |
| Analyzing Compounds | Monocrotophos, Metribuzin, Bromacil, Carbofuran, 4-Chlorophenoxyacetic acid, Bentazone, Folpet, Fenthion, Fenvalerate, Metrabuzin, Carbofuran, Atrazine, Parathion –methyl, MCPA, Clopyralid, Dicamba, Benthazon, Simazine, Diuron, MCPB, Mecoprop, 4-Chlorophenoxyacetic acid,2,4,5-T |
Application Analytes:
2,4,5-Trichlorophenoxyacetic acid
4-CPA
Atrazine
Bentazone-sodium
Carbofuran
Clopyralid
Dicamba
Diuron
Fenvalerate
Folpet
MCPA
MCPB
Mecoprop
Methyl parathion
Monocrotophos
Simazine