Methylparaben

CAS Number 99-76-3
Molecular Formula C8H8O3
Molecular Weight 152.150 g/mol
InChI Key LXCFILQKKLGQFO-UHFFFAOYSA-N
LogP 1.96
Synonyms
  • Methylparaben
  • Methyl 4-hydroxybenzoate
  • Benzoic acid, 4-hydroxy-, methyl ester
  • 99-76-3
  • Methyl paraben (Benzoic acid, 4-hydroxy-, methyl ester)
  • Benzoic acid, 4-hydroxy-, methyl ester
  • 4-(Carbomethoxy)phenol
  • 4-(Methoxycarbonyl)phenol
  • 4-hidroxibenzoato de metilo
  • 4-Hydroxybenzoate de methyle
  • 4-HYDROXYBENZOESAEURE-METHYLESTER
  • 4-Hydroxybenzoic acid methyl ester
  • BENZOATE, 4-HYDROXY-, METHYL
  • Benzoic acid, p-hydroxy-, methyl ester
  • Danisol M
  • Killitol
  • Maseptol
  • Mekkings M
  • Metaben
  • Metagin
  • Methaben
  • methyl 4-hydroxybenzoate
  • Methyl Butex
  • Methyl chemosept
  • METHYL ESTER OF P-HYDROXY BENZOIC ACID
  • METHYL PARABEN
  • Methyl Parasept
  • METHYL P-HYDROXYBENZOATE
  • Methyl-4-hydroxybenzoat
  • Methylben
  • Metoxyde
  • Nipagin
  • Nipagin M
  • NSC 3827
  • NSC 406127
  • Paraben M
  • PARIDOL
  • p-Carbomethoxyphenol
  • P-HYDROXYBENZOIC ACID METHYL ESTER
  • p-Methoxycarbonylphenol
  • Preserval
  • Preserval M
  • Solbrol
  • Solbrol M
  • Tegosept M
  • Aseptoform
  • BRN 0509801
  • Caswell No. 573PP
  • EINECS 202-785-7
  • EPA Pesticide Chemical Code 061201
  • FEMA Number 2710
  • Methylester kyseliny p-hydroxybenzoove
  • UNII-A2I8C7HI9T
  • E 218
  • E218
  • FEMA No. 2710
  • INS No. 218
  • INS number 218
  • Methyl ester of p-hydroxybenzoic acid
  • Methyl p-oxybenzoate
  • Methyl parahydroxybenzoate
  • Methyl-p-hydroxybenzoate
  • P-Hydroxybenzoic acid, methyl ester
  • P-Hydroxybenzoic methyl ester
  • p-Oxybenzoesauremethylester
  • p-methoxycarbonylphenol
  • 1000398-37-7
  • 156291-94-0
  • 58339-84-7

Applications:


HPLC Separation of Parabens and Benzoic Acid

Parabens possess antibacterial and antifungal properties and are therefore widely used in pharmaceutical and cosmetic industries as preservatives in products. Parabens and benzoic acid can be baseline separated in a short time frame using Primesep B2 reverse-phase HPLC column with a simple mobile phase of water, acetonitrile (ACN, MeCN) and phosphoric acid of 0.1% as buffer. UV detection at 210nm.

Application Analytes:

Benzoic Acid
Methylparaben
Parabens
Propylparaben

HPLC Analysis of Active Drug and Amino Acids in a Formulation
Polar amino acids are very often used as components of vitamin and supplement composition. Analysis of such complex composition is a challenging task. In this application, 5 amino acids (asparagine, glutamic acid, proline and arginine) and two preservatives (methyl paraben and propyl paraben) are separated on a Primesep 100 reversed-phase cation-exchange column with LC/MS compatible mobile phase. Method does not require ion-pairing reagent in the mobile phase. Compounds are monitored by ELSD and UV. Method is validated for quantitation of underivatized amino acids in complex mixtures. The method is simple and robust and can be used for analysis of various vitamin formulations.

Condition

Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm
Flow Rate 1.0 ml/min
Detection ELSD 50C, UV 250 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Glutamic acid, Aspargine,  Proline, Lysine, Arginine, Methyl paraben, Propyl paraben
 

Application Analytes:

Arginine
Asparagine
Ethyl Paraben
Glutamic Acid
Lysine
Methylparaben
Proline

HPLC Separation of Methyl Paraben, Benzonitrile, Propyl Paraben, and Toluene on Mixed-Mode and Reverse Phase Columns


Parabens are common preservatives in pharmaceutical and cosmetic industries. They are esters of p-hydroxybenzoic acid. Method for separation of methyl paraben, propyl paraben, benzonitrile and toluene was developed on a Obelisc R column. All four compounds are neutral and are retained by reverse-phase mechanism. In case of reversed-phase stationary phase, no effect of pH is observed. Retention time for all four compounds changes on an Obelisc R column when pH is changed. pH of the mobile phase affects ionization state of stationary phase. Obelisc R column has C12 carbon chain and carboxylic acid with pKa of 4. At lower pH (pH 2, TFA), carboxylic acid of stationary phase is not ionized and thus adds hydrophobicity to stationary phase. Obelisc R column can be used for analysis of basic, acidic and neutral compounds with suitable detection techniques - UV, ELSD, CAD, LC/MS.



Application Analytes:

Benzonitrile
Methylparaben
Propylparaben
Toluene

Analysis of Codeine-Based Drug Composition. Effect of Buffer Concentration and Buffer pH

Codeine and two parabens are separated by mixed-mode chromatography. Codeine is retained by reversed-phase and cation-exchange mechanisms and parabens are retained by reversed-phase mechanism. The retention time of codeine can be adjusted by changing the amount of acetonitrile, buffer concentration, and buffer pH. The mobile phase for this column and its separation are fully compatible with UV, ELSD, LC/MS and prep chromatography, various organic and inorganic acids, and corresponding buffers can be used.

Codeine is a hydrophobic basic drug which is used in many drug compositions as an analgetic, antitussive, anxiolytic, and sedative agent. Codeine is widely used as a moderate pain and cough reliever. It is usually part of complex composition and comes in the form of a tablet or syrup. Several preservatives are used in most of the drug composition and include parabens and benzoates. The mixture of codeine, methyl and propyl parabens was separated on Primesep C mixed-mode reversed-phase cation-exchange column. Codeine is retained by reversed-phase and cation-exchange mechanisms and parabens are retained by reversed-phase mechanism. No ion-pairing reagent is required since Primesep C mixed-mode stationary phase has an ion-pairing reagent attached to the surface.



Application Analytes:

Codeine
Methylparaben
Propylparaben

Analysis off Codeine-based Drug Composition. Effect of Buffer Concentration and Buffer PH

Condition

Column Primesep C, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 35/65%
Buffer TEAPh Ph 3.5 - 15 Mm
Flow Rate 1.0 ml/min
Detection UV, 210 nm
 

Description

Class of Compounds Drug, Preservatives,  Base, Hydrophobic, Ionizable
Analyzing Compounds Codeine, Methyl Paraben, Propyl Paraben
 

Application Analytes:

Codeine
Methylparaben
Propylparaben

Analysis of Codeine and Related Impurities in Drug Composition

Condition

Column Primesep C, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 35/65%
Buffer TEAPh Ph 3.5 - 15 Mm
Flow Rate 1.0 ml/min
Detection UV, 210 nm
 

Description

Class of Compounds Drug, Preservatives,  Base, Hydrophobic, Ionizable
Analyzing Compounds Codeine, Methyl Paraben, Propyl Paraben
 

Application Analytes:

Codeine
Methylparaben
Propylparaben

Analysis of Dextromethorphan-Based Composition
 

Condition

Column Primesep C, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TEAPh
Flow Rate 1.0 ml/min
Detection UV, 210 nm
 

Description

Class of Compounds Drug, Preservatives,  Base, Hydrophobic, Ionizable
Analyzing Compounds Dextromethorphan, Bromide, Methyl Paraben, Propyl Paraben
 

Application Analytes:

Bromide
Dextromethorphan
Methylparaben
Propylparaben

Analysis of Dextromethorphan-Based Drug composition. Effect on buffer pH
Dextromethorphan is one of the common cough suppressants used in many drug composition. It is in tablets and syrups as an antitussive drug. Composition often has preservatives like parabens. Dextromethorphan is a hydrophobic, basic drug which is used as a bromide salt in drug compositions. Dextromethorphan and two parabens (methyl paraben and propyl paraben) were separated on Primesep C reversed-phase cation-exchange column. Several impurities were observed and are well separated from the main components of the drug composition. Method can be used for various formulations in QC and production environment.  

Condition

Column Primesep C, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 35/65%
Buffer TEAPh
Flow Rate 1.0 ml/min
Detection UV, 210 nm
 

Description

Class of Compounds Drug, Preservatives,  Base, Hydrophobic, Ionizable
Analyzing Compounds Dextromethorphan, Bromide, Methyl Paraben, Propyl Paraben
 

Application Analytes:

Bromide
Dextromethorphan
Methylparaben
Propylparaben

Generic Screening Method for Complex Mixtures

Condition

Column Primesep 200, 4.6*150 mm 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H2SO4
Flow Rate 1.0 ml/min
Detection UV, 215 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Hormone
Analyzing Compounds Uracil, Epinephrine, DOPA, 2,6-Lutidine, Benzylamine, Hydroxytrypthophan, Homovanillic acid, Phenol, Tryptophan , 2,3-DHBA, Benzoic acid, Methylparaben, Ethylparaben, Toluene, Amitriptyline
 

Application Analytes:

2,3-Dihydroxybenzoic Acid
2,6-Lutidine
Amitriptyline
Benzoic Acid
Benzylamine
DOPA (3,4-dihydroxy-L-phenylalanine)
Epinephrine
Ethyl Paraben
Homovanillic Acid
Hydroxytryptophan
Methylparaben
Phenol
Toluene
Tryptophan
Uracil

HPLC Separation of Mixture of Carbocisteine, Methylparaben & Propylparaben on Primesep 100 Column
 

Condition

Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN  - 2-50%, 20 min
Buffer H2SO4 - 0.05 %
Flow Rate 1.0 ml/min
Detection UV, 200 nm
 

Description

Class of Compounds Drug,  Acid, Hydrophilic, Ionizable, Carboxylic acid, Carbocyclic, Benzoate, Hydroxy Acid, Amino Acid, Hydroxybenzoate, Phenol
Analyzing Compounds Carbocisteine, Methylparaben, Propylparaben
 

Application Analytes:

Carbocisteine
Methylparaben
Methylparaben sodium
Propylparaben
Propylparaben sodium