Methylparaben

Methyl Paraben structural formula

CAS Number99-76-3
Molecular FormulaC8H8O3
Molecular Weight152.150
InChI KeyLXCFILQKKLGQFO-UHFFFAOYSA-N
LogP1.96
Synonyms
  • Methylparaben
  • Methyl 4-hydroxybenzoate
  • Benzoic acid, 4-hydroxy-, methyl ester
  • 99-76-3
  • Methyl paraben (Benzoic acid, 4-hydroxy-, methyl ester)
  • Benzoic acid, 4-hydroxy-, methyl ester
  • 4-(Carbomethoxy)phenol
  • 4-(Methoxycarbonyl)phenol
  • 4-hidroxibenzoato de metilo
  • 4-Hydroxybenzoate de methyle
  • 4-HYDROXYBENZOESAEURE-METHYLESTER
  • 4-Hydroxybenzoic acid methyl ester
  • BENZOATE, 4-HYDROXY-, METHYL
  • Benzoic acid, p-hydroxy-, methyl ester
  • Danisol M
  • Killitol
  • Maseptol
  • Mekkings M
  • Metaben
  • Metagin
  • Methaben
  • methyl 4-hydroxybenzoate
  • Methyl Butex
  • Methyl chemosept
  • METHYL ESTER OF P-HYDROXY BENZOIC ACID
  • METHYL PARABEN
  • Methyl Parasept
  • METHYL P-HYDROXYBENZOATE
  • Methyl-4-hydroxybenzoat
  • Methylben
  • Metoxyde
  • Nipagin
  • Nipagin M
  • NSC 3827
  • NSC 406127
  • Paraben M
  • PARIDOL
  • p-Carbomethoxyphenol
  • P-HYDROXYBENZOIC ACID METHYL ESTER
  • p-Methoxycarbonylphenol
  • Preserval
  • Preserval M
  • Solbrol
  • Solbrol M
  • Tegosept M
  • Aseptoform
  • BRN 0509801
  • Caswell No. 573PP
  • EINECS 202-785-7
  • EPA Pesticide Chemical Code 061201
  • FEMA Number 2710
  • Methylester kyseliny p-hydroxybenzoove
  • UNII-A2I8C7HI9T
  • E 218
  • E218
  • FEMA No. 2710
  • INS No. 218
  • INS number 218
  • Methyl ester of p-hydroxybenzoic acid
  • Methyl p-oxybenzoate
  • Methyl parahydroxybenzoate
  • Methyl-p-hydroxybenzoate
  • P-Hydroxybenzoic acid, methyl ester
  • P-Hydroxybenzoic methyl ester
  • p-Oxybenzoesauremethylester
  • p-methoxycarbonylphenol
  • 1000398-37-7
  • 156291-94-0
  • 58339-84-7

Applications:

HPLC Analysis of Paraben Preservatives on Newcrom AH Column

October 29, 2020


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Condition

Column Newcrom AH, 3.2×100 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 30/70%
Buffer H2SO4 – 0.1%
Flow Rate 0.5 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
Preservatives,  Hydrophobic
Analyzing Compounds Methyl Paraben, Ethyl Paraben, Propyl Paraben

 

Application Column

Newcrom AH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Ethylparaben
Methylparaben
Methylparaben sodium
Parabens
Propylparaben
Propylparaben sodium
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Determination of EDTA, Phenoxyethanol and Methylparaben in Facial Cleanser on Newcrom BH

August 25, 2020


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Condition

Column Newcrom BH, 4.6×150 mm,  100A
Mobile Phase Gradient MeCN – 2- 70%, 10 min
Buffer H2SO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV 260nm

Description

Class of Compounds Acid, Hydrophilic, Preservatives
Analyzing Compounds EDTA, Methylparaben, Phenoxyethanol

 

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
2-Phenoxyethanol
EDTA (Ethylenediaminetetraacetic Acid)
Methylparaben
Methylparaben sodium
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Mixture of Carbocisteine, Methylparaben & Propylparaben on Primesep 100 Column

July 31, 2019


Carbocysteine is a medication that reduces the viscosity of mucus making it easier to cough up. Methylparaben and propylparaben are used as preservatives in cosmetics, pharmaceuticals and food. They can be separated in HPLC on a Primesep 100 mixed-mode column by both hydrophobic and ionic properties present on the stationary phase. The analytical method uses acetonitrile (ACN) gradient and water with sulfuric acid (H2SO4) as buffer and UV detected at 200nm.

Condition

Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN  – 2-50%, 20 min
Buffer H2SO4 – 0.05 %
Flow Rate 1.0 ml/min
Detection UV, 200 nm

 

Description

Class of Compounds
Drug,  Acid, Hydrophilic, Ionizable, Carboxylic acid, Carbocyclic, Benzoate, Hydroxy Acid, Amino Acid, Hydroxybenzoate, Phenol
Analyzing Compounds Carbocisteine, Methylparaben, Propylparaben

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Carbocisteine
Methylparaben
Methylparaben sodium
Propylparaben
Propylparaben sodium
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Generic Screening Method for Complex Mixtures

October 15, 2015

Condition

Column Primesep 200, 4.6*150 mm 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H2SO4
Flow Rate 1.0 ml/min
Detection UV, 215 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Hormone
Analyzing Compounds Uracil, Epinephrine, DOPA, 2,6-Lutidine, Benzylamine, Hydroxytrypthophan, Homovanillic acid, Phenol, Tryptophan , 2,3-DHBA, Benzoic acid, Methylparaben, Ethylparaben, Toluene, Amitriptyline

 

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
2,3-Dihydroxybenzoic Acid
2,6-Lutidine
Amitriptyline
Benzoic Acid
Benzylamine
DOPA (3,4-dihydroxy-L-phenylalanine)
Epinephrine
Ethylparaben
Homovanillic Acid
Hydroxytryptophan
Methylparaben
Phenol
Toluene
Tryptophan
Uracil

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Analysis of Codeine-based Drug Composition. Effect of Buffer Concentration and Buffer PH

July 3, 2013

Condition

Column Primesep C, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 35/65%
Buffer TEAPh Ph 3.5 – 15 Mm
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
Drug, Preservatives,  Base, Hydrophobic, Ionizable
Analyzing Compounds Codeine, Methyl Paraben, Propyl Paraben

 

Application Column

Primesep C

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Codeine
Methylparaben
Propylparaben

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Analysis of Codeine and Related Impurities in Drug Composition

July 3, 2013

Condition

Column Primesep C, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 35/65%
Buffer TEAPh Ph 3.5 – 15 Mm
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
Drug, Preservatives,  Base, Hydrophobic, Ionizable
Analyzing Compounds Codeine, Methyl Paraben, Propyl Paraben

 

Application Column

Primesep C

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Codeine
Methylparaben
Propylparaben

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Analysis of Dextromethorphan-Based Composition

July 3, 2013

 

Condition

Column Primesep C, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TEAPh
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
Drug, Preservatives,  Base, Hydrophobic, Ionizable
Analyzing Compounds Dextromethorphan, Bromide, Methyl Paraben, Propyl Paraben

 

Application Column

Primesep C

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Bromide
Dextromethorphan
Methylparaben
Propylparaben

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Analysis of Dextromethorphan-Based Drug composition. Effect on buffer pH

July 3, 2013

Dextromethorphan is one of the common cough suppressants used in many drug composition. It is in tablets and syrups as an antitussive drug. Composition often has preservatives like parabens. Dextromethorphan is a hydrophobic, basic drug which is used as a bromide salt in drug compositions. Dextromethorphan and two parabens (methyl paraben and propyl paraben) were separated on Primesep C reversed-phase cation-exchange column. Several impurities were observed and are well separated from the main components of the drug composition. Method can be used for various formulations in QC and production environment.

 

Condition

Column Primesep C, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 35/65%
Buffer TEAPh
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
Drug, Preservatives,  Base, Hydrophobic, Ionizable
Analyzing Compounds Dextromethorphan, Bromide, Methyl Paraben, Propyl Paraben

 

Application Column

Primesep C

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Bromide
Dextromethorphan
Methylparaben
Propylparaben

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Analysis of Codeine-Based Drug Composition. Effect of Buffer Concentration and Buffer pH

July 2, 2013

Codeine and two parabens are separated by mixed-mode chromatography. Codeine is retained by reversed-phase and cation-exchange mechanisms and parabens are retained by reversed-phase mechanism. The retention time of codeine can be adjusted by changing the amount of acetonitrile, buffer concentration, and buffer pH. The mobile phase for this column and its separation are fully compatible with UV, ELSD, LC/MS and prep chromatography, various organic and inorganic acids, and corresponding buffers can be used.

Codeine is a hydrophobic basic drug which is used in many drug compositions as an analgetic, antitussive, anxiolytic, and sedative agent. Codeine is widely used as a moderate pain and cough reliever. It is usually part of complex composition and comes in the form of a tablet or syrup. Several preservatives are used in most of the drug composition and include parabens and benzoates. The mixture of codeine, methyl and propyl parabens was separated on Primesep C mixed-mode reversed-phase cation-exchange column. Codeine is retained by reversed-phase and cation-exchange mechanisms and parabens are retained by reversed-phase mechanism. No ion-pairing reagent is required since Primesep C mixed-mode stationary phase has an ion-pairing reagent attached to the surface.

Application Column

Primesep C

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Codeine
Methylparaben
Propylparaben

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Analysis of Active Drug and Amino Acids in a Formulation

October 14, 2010


Polar amino acids are very often used as components of vitamin and supplement composition. Analysis of such complex composition is a challenging task. In this application, 5 amino acids (asparagine, glutamic acid, proline and arginine) and two preservatives (methyl paraben and propyl paraben) are separated on a Primesep 100 reversed-phase cation-exchange column with LC/MS compatible mobile phase. Method does not require ion-pairing reagent in the mobile phase. Compounds are monitored by ELSD and UV. Method is validated for quantitation of underivatized amino acids in complex mixtures. The method is simple and robust and can be used for analysis of various vitamin formulations.

Condition

Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm
Flow Rate 1.0 ml/min
Detection ELSD 50C, UV 250 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Glutamic acid, Aspargine,  Proline, Lysine, Arginine, Methyl paraben, Propyl paraben

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Arginine
Asparagine
Ethylparaben
Glutamic Acid
Lysine
Methylparaben
Proline

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Methyl Paraben, Benzonitrile, Propyl Paraben, and Toluene on Mixed-Mode and Reverse Phase Columns

October 4, 2010


Parabens are common preservatives in pharmaceutical and cosmetic industries. They are esters of p-hydroxybenzoic acid. Method for separation of methyl paraben, propyl paraben, benzonitrile and toluene was developed on a Obelisc R column. All four compounds are neutral and are retained by reverse-phase mechanism. In case of reversed-phase stationary phase, no effect of pH is observed. Retention time for all four compounds changes on an Obelisc R column when pH is changed. pH of the mobile phase affects ionization state of stationary phase. Obelisc R column has C12 carbon chain and carboxylic acid with pKa of 4. At lower pH (pH 2, TFA), carboxylic acid of stationary phase is not ionized and thus adds hydrophobicity to stationary phase. Obelisc R column can be used for analysis of basic, acidic and neutral compounds with suitable detection techniques – UV, ELSD, CAD, LC/MS.

Condition

Column Obelisc R, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc, TFA
Flow Rate 1.0 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
Preservatives, Neutral
Analyzing Compounds Methylparaben, Benzonitrile, Propyl paraben, Toluene

 

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

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Application Analytes:
Benzonitrile
Methylparaben
Propylparaben
Toluene

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Parabens and Benzoic Acid

June 5, 2006


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Condition 1

Column Newcrom B, 3.2×100 mm, 3 µm, 100A
Mobile Phase MeCN/H2O – 40/60%
Buffer
Flow Rate 0.5 ml/min
Detection UV 275nm

 


Parabens possess antibacterial and antifungal properties and are therefore widely used in pharmaceutical and cosmetic industries as preservatives in products. Parabens and benzoic acid can be baseline separated in a short time frame using Primesep B2 reverse-phase HPLC column with a simple mobile phase of water, acetonitrile (ACN, MeCN) and phosphoric acid of 0.1% as buffer. UV detection at 210nm.

Condition 2

Column Primesep B2, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H3PO4
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
Acid, Hydrophilic, Preservative
Analyzing Compounds Methyl paraben, Benzoic Acid, Propyl paraben

 

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Primesep B2

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Benzoic Acid
Methylparaben
Parabens
Propylparaben

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.