Methylene Blue

CAS Number 61-73-4
Molecular Formula C16H18ClN3S
Molecular Weight 319.851 g/mol
InChI Key CXKWCBBOMKCUKX-UHFFFAOYSA-M
LogP 2.72
Synonyms
  • Methylene blue
  • 3,7-Bis(dimethylamino)phenothiazin-5-ium chloride
  • Phenothiazinium, 3,7-bis(dimethylamino)-, chloride (1:1)
  • 61-73-4
  • Phenothiazin-5-ium, 3,7-bis(dimethylamino)-, chloride
  • 3,7-BIS(DIMETHYLAMINO)PHENAZATHIONIUM CHLORIDE
  • 3,7-Bis(dimethylamino)phenothiazin-5-ium chloride
  • C.I. basic blue 009
  • Chlorure de methylthioninium
  • Chromosmon
  • cloruro de metiltioninio
  • Izit Crystal Dye
  • METHYLENBLAU
  • Methylene blue polychrome
  • Methylenium ceruleum
  • METHYLTHIONINE CHLORIDE
  • methylthioninium chloride
  • Methylthioniniumchlorid
  • NSC 617593
  • Phenothiazin-5-ium, 3,7-bis(dimethylamino)-, chloride (1:1)
  • Schultz 1038
  • Swiss blue
  • Tetramethylthionine
  • Tetramethylthionine chloride
  • D and C Blue No. 1
  • D And C Blue Number 1
  • C.I. Basic Blue 9
  • M-B Tabs
  • Aizen Methylene Blue BH
  • Aizen Methylene Blue FZ
  • Calcozine Blue ZF
  • Caswell No. 567
  • Ceruleum methylenum
  • CI Basic Blue 9
  • EINECS 200-515-2
  • EPA Pesticide Chemical Code 039505
  • Ext D and C Blue No. 1
  • External Blue 1
  • Hidaco Methylene Blue Salt Free
  • Leather Pure Blue HB
  • Methylene Blue A
  • Methylene Blue B
  • Methylene Blue D
  • Methylene Blue G
  • Methylene Blue N
  • Methylene Blue 2B
  • Methylene Blue 2BF
  • Methylene Blue 2BN
  • Methylene Blue 2BP
  • Methylene Blue BB
  • Methylene Blue BBA
  • Methylene Blue BD
  • Methylene Blue BP
  • Methylene Blue BPC
  • Methylene Blue BX
  • Methylene Blue BZ
  • Methylene Blue chloride
  • Methylene Blue FZ
  • Methylene Blue GZ
  • Methylene Blue HGG
  • Methylene Blue IAD
  • Methylene Blue JFA
  • Methylene Blue NZ
  • Methylene Blue SG
  • Methylene Blue SP
  • Methylene Blue ZF
  • Methylene Blue Zinc Free
  • Methylene Blue ZX
  • Methylenum coeruleum
  • Methylthionini chloridum
  • Methylthionium chloride
  • Mitsui Methylene Blue
  • NSC 215213
  • Sandocryl Blue BRL
  • Schultz No. 1038
  • Tetramethylene Blue
  • Urolene Blue
  • Yamamoto Methylene Blue B
  • Yamamoto Methylene Blue ZF
  • Methylthioninii chloridum
  • Metiltioninio cloruro
  • Modr Methylenova
  • Modr Rozpoustedlova 8
  • Modr Zasadita 9
  • UNII-8NAP7826UB
  • Basic Blue 9
  • C.I. 52015
  • Methylene Blue anhydrous
  • Solvent blue 8
  • azul de metileno
  • bleu de methylene
  • 152071-32-4
  • 1001913-24-1
  • 1001914-35-7
  • 105504-42-5
  • 121067-62-7
  • 1219638-11-5
  • 12262-49-6
  • 1341-90-8
  • 167498-52-4
  • 6476-03-5
  • 923927-02-0
  • 97130-83-1

Applications:


HPLC Separation of Dyes


Various dyes are widely used in food, textile and pharmaceutical industries. Dies are aromatic compounds containing ionizable groups. Methylene blue, crystal violet lactone and crystal violet were separated on a Primesep D HPLC column. Method can be used for separation of other dyes and pigments that are basic and hydrophobic in nature.



Application Analytes:

Crystal Violet
Crystal Violet Lactone
Methylene Blue

HPLC Dye Separation on SHARC1 Column
 

Condition

Column Sharc 1, 3.2x100 mm, 3 µm, 100A
Mobile Phase MeCN/EtOH - 99/1%
Buffer H2SO4 - 0.5%
Flow Rate 1.5 ml/min
Detection UV, 500, 650 nm
 

Description

Class of Compounds Dye, Hydrophilic, Ionizable
Analyzing Compounds Methyl Red, Methyl Orange, Methylene Blue, Nile Blue A
 

Application Analytes:

Methyl Orange
Methyl Red
Methylene Blue
Nile Blue A

Analysis of Methylene Blue Purity in Reversed-Phase Cation-Exchange and Reversed-Phase Cation-Exclusion Modes
Methylene blue is a heterocyclic dye with a wide range of use in biology and chemistry. Purity of dyes rarely exceed 70% and separation of methylene blue and related impurities is required. Two alternative methods for purity analysis were developed on Primesep C and Primesep D mixed-mode HPLC columns. Primesep C is a reversed-phase cation-exchange (anion-exclusion column) column and Primesep D is reversed-phase anion-exchange (cation-exclusion) column. Various buffers can be used for the method. Depending on the buffer, method can be compatible with UV, LC/MS and prep chromatography. Method can be used for prep separation of methylene blue and related impurities.

Condition 1

Column Primesep D, 4.6x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 5-20%
Buffer Gradient NaH2PO4 pH 2.5 70-20mM 15 min
Flow Rate 1.0 ml/min
Detection UV, 300 nm
> The antibiotic norfloxacin is a first generation fluoroquinolone. It is effective against both gram positive and gram negative bacteria. Analysis was done on Obelisc R and Primesep D.  

Condition 2

Column Primesep C, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN - 35%
Buffer AmFm pH 3.5 - 35 mM
Flow Rate 1.0 ml/min
Detection UV, 300 nm
 

Description

Class of Compounds  Duy, Hydrophobic, Ionizable
Analyzing Compounds  Methylene Blue
 

Application Analytes:

Methylene Blue