Methylmalonic Acid

CAS Number 516-05-2
Molecular Formula C4H6O4
Molecular Weight 118.088 g/mol
LogP -0.367
  • Methylmalonic acid
  • Methylpropanedioic acid
  • Propanedioic acid, 2-methyl-
  • 516-05-2
  • 4-02-00-01932
  • Propanedioic acid, methyl-
  • 1,1-Ethanedicarboxylic acid
  • 2-Methylmalonic acid
  • acide methylmalonique
  • acido metilmalonico
  • Isosuccinic acid
  • Malonic acid, methyl-
  • Methylmalonsaeure
  • Methylpropanedioic acid
  • NSC 25201
  • Propanedioic acid, 2-methyl-
  • BRN 1756084
  • EINECS 208-219-5
  • UNII-8LL8S712J7
  • 1,1-Ethanedicarboxylate
  • 2-Methylmalonate
  • 2-methylpropanedioic acid
  • Isosuccinate
  • Methyl-Malonate
  • Methyl-Malonic acid
  • Methyl-Propanedioate
  • Methyl-Propanedioic acid
  • Methylmalonate
  • Methylpropanedioate
  • alpha-methylmalonic acid


HPLC Separation of Organic Acids in HILIC Mode on Primesep N Column

Ascorbic, methylmalonic and succinic are weak organic acids. Retention of these three acids is achieved on Primesep N column in HILIC mode using acetonitrile/water and ammonium acetate. Compounds are monitored by ELSD. Method can be used for determination of ascorbic acid (Vitamin C), methylmalonic acid and succinic acid in various matrices. Other polar organic acids can be analyzed on this HILIC column.

Application Analytes:

Ascorbic Acid
Methylmalonic Acid
Organic Acids
Succinic Acid

HPLC Separation of Succinic and Methylmalonic Acids

Organic and inorganic acids can be separated by reversed-phase anion-exchange Primesep D column. Succinic and methylmalonic acids were separated based on their hydrophobic and ionic properties. Method can be used for analysis of other acids by HPLC.

Application Analytes:

Methylmalonic Acid
Organic Acids
Succinic Acid

HILIC Separation of Carboxylic Acids

Hydrophilic acids are separated on Obelisc N mixed-mode HILIC column. Seven carboxylic acids are separated based on their polarity and pKa values. Changes in ionization states of acids and stationary phase can be used to control elution order of organic and inorganic acids.

Application Analytes:

Fumaric Acid
Hydroxybenzoic Acid
Malic Acid
Mandelic Acid
Methylmalonic Acid
Organic Acids
Succinic Acid
Tartaric Acid