Methylmalonic Acid

CAS Number 516-05-2
Molecular Formula C4H6O4
Molecular Weight 118.088 g/mol
InChI Key ZIYVHBGGAOATLY-UHFFFAOYSA-N
LogP -0.367
Synonyms
  • Methylmalonic acid
  • Methylpropanedioic acid
  • Propanedioic acid, 2-methyl-
  • 516-05-2
  • 4-02-00-01932
  • Propanedioic acid, methyl-
  • 1,1-Ethanedicarboxylic acid
  • 2-Methylmalonic acid
  • acide methylmalonique
  • acido metilmalonico
  • Isosuccinic acid
  • Malonic acid, methyl-
  • Methylmalonsaeure
  • Methylpropanedioic acid
  • NSC 25201
  • Propanedioic acid, 2-methyl-
  • BRN 1756084
  • EINECS 208-219-5
  • UNII-8LL8S712J7
  • 1,1-Ethanedicarboxylate
  • 2-Methylmalonate
  • 2-methylpropanedioic acid
  • Isosuccinate
  • Methyl-Malonate
  • Methyl-Malonic acid
  • Methyl-Propanedioate
  • Methyl-Propanedioic acid
  • Methylmalonate
  • Methylpropanedioate
  • alpha-methylmalonic acid

Applications:


HPLC Separation of Organic Acids in HILIC Mode on Primesep N Column



Ascorbic, methylmalonic and succinic are weak organic acids. Retention of these three acids is achieved on Primesep N column in HILIC mode using acetonitrile/water and ammonium acetate. Compounds are monitored by ELSD. Method can be used for determination of ascorbic acid (Vitamin C), methylmalonic acid and succinic acid in various matrices. Other polar organic acids can be analyzed on this HILIC column.



Application Analytes:

Ascorbic Acid
Methylmalonic Acid
Organic Acids
Succinic Acid

HPLC Separation of Succinic and Methylmalonic Acids


Organic and inorganic acids can be separated by reversed-phase anion-exchange Primesep D column. Succinic and methylmalonic acids were separated based on their hydrophobic and ionic properties. Method can be used for analysis of other acids by HPLC.



Application Analytes:

Methylmalonic Acid
Organic Acids
Succinic Acid

HILIC Separation of Carboxylic Acids



Hydrophilic acids are separated on Obelisc N mixed-mode HILIC column. Seven carboxylic acids are separated based on their polarity and pKa values. Changes in ionization states of acids and stationary phase can be used to control elution order of organic and inorganic acids.



Application Analytes:

Fumaric Acid
Hydroxybenzoic Acid
Malic Acid
Mandelic Acid
Methylmalonic Acid
Organic Acids
Succinic Acid
Tartaric Acid

HPLC Separation of Small Organic Acids on Newcrom B Column
HPLC.cloud View on hplc.cloud  

Condition

Column Newcrom B, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 5/95%
Buffer AmAc pH 5.0, Formic Acid
Flow Rate 1.0 ml/min
Detection CAD (Corona) MS- compatible mobile phase

Description

Class of Compounds Acid, Hydrophilic, Ionizable
Analyzing Compounds Glycolic acid, Succinic Acid, Methylmalonic acid, Malic Acid, Maleic Acid, Citric Acid, Tartaric Acid, Fumaric Acid, Malonic Acid


Application Analytes:

Citric Acid
Fumaric Acid
Glycolic acid sodium salt
Maleic Acid
Malic Acid
Malonic Acid
Methylmalonic Acid
Succinic Acid
Tartaric Acid
dl-Tartaric acid

HPLC Separation of Methylmalonic, Fumaric, Maleic acids on Newcrom B Column

Condition

Column Newcrom B, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeOH/H2O - 5/95%
Buffer Formic Acid - 2%
Flow Rate 1.0 ml/min
Detection CAD (Corona)  (MS-compatible mobile phase)

Description

Class of Compounds Acid, Hydrophilic, Ionizable
Analyzing Compounds Methylmalonic acid, Fumaric acid, Maleic acid
 

Application Analytes:

Fumaric Acid
Maleic Acid
Methylmalonic Acid

HPLC Separation of Methylmalonic and Malonic Acid on Newcrom B Column
HPLC Separation of Methylmalonic and Malonic Acid on Newcrom B Column_1199

Condition

Column Newcrom B, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 10/90%
Buffer H3PO4 - 0.02%
Flow Rate 1.0 ml/min
Detection UV 200nm

Description

Class of Compounds Acid, Hydrophilic, Polar
Analyzing Compounds Methylmalonic acid,  Malonic Acid


Application Analytes:

Malonic Acid
Methylmalonic Acid