| CAS Number | 98-92-0 |
|---|---|
| Molecular Formula | C6H6N2O |
| Molecular Weight | 122.127 g/mol |
| InChI Key | DFPAKSUCGFBDDF-UHFFFAOYSA-N |
| LogP | -0.370 |
| Synonyms |
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Applications:
Separation of Nicotinamide and Related Substances
A complex mixture of nicotinamide and related impurities was separated on Obelisc R mixed-mode column. Nicotinamide, methylnicotinamide, nicotinamide adenine mononucleotide, nicotinamide adenine dinucleotide, and adenosine monophosphate were baseline resolved in a 15 minute long method. This mixed-mode approach can be used for analysis of other nucleotides. Obelisc R trimodal column separates this complex mixture based on reversed-phase, cation-exchange and anion-exchange mechanisms. Retention is controlled by amount of ACN, buffer concentration and buffer pH. Additional selectivity can be gained by exploring various buffers within the same pH
Application Analytes:
1-Methylnicotinamide
Adenosine Monophosphate
Nicotinamide
Nicotinamide Adenine Dinucleotide
Nicotinamide Adenine Mononucleotide
Analysis of Nicotinic Acid in Nicotinamide
Nicotinamide is the amide form of nicotinic acid. Nicotinic acid is in the vitamin B group and is converted to nicotinamide in vivo. Primesep 100 was used to separate nicotinamide and nicotinic acid. Concentrations of nicotinic acid as low as 0.1ppm could be used.Primesep 100 separates acids using ion-exclusion, retains basic compounds by cation-exchange, and neutral compounds by reverse-phase mechanism.
| Column | Primesep 100, 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O - 5/95% |
| Buffer | Na2HPO4 pH 3.0- 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 230 nm |
| Class of Compounds | Acid, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Nicotinic Acid, Nicotinamide |
Application Analytes:
Niacin
Nicotinamide
Nicotinic Acid