| CAS Number | 98-92-0 |
|---|---|
| Molecular Formula | C6H6N2O |
| Molecular Weight | 122.127 g/mol |
| InChI Key | DFPAKSUCGFBDDF-UHFFFAOYSA-N |
| LogP | -0.370 |
| Synonyms |
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Applications:
Separation of Nicotinamide and Related Substances
A complex mixture of nicotinamide and related impurities was separated on Obelisc R mixed-mode column. Nicotinamide, methylnicotinamide, nicotinamide adenine mononucleotide, nicotinamide adenine dinucleotide, and adenosine monophosphate were baseline resolved in a 15 minute long method. This mixed-mode approach can be used for analysis of other nucleotides. Obelisc R trimodal column separates this complex mixture based on reversed-phase, cation-exchange and anion-exchange mechanisms. Retention is controlled by amount of ACN, buffer concentration and buffer pH. Additional selectivity can be gained by exploring various buffers within the same pH
Application Analytes:
1-Methylnicotinamide
Adenosine Monophosphate
Nicotinamide
Nicotinamide Adenine Dinucleotide (NAD)
Nicotinamide Adenine Mononucleotide
Analysis of Nicotinic Acid and Nicotinamide (Vitamin B3) on Newcrom AH Column
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Nicotinamide is the amide form of nicotinic acid. Nicotinic acid is in the vitamin B group and is converted to nicotinamide in vivo. Newcrom AH was used to separate nicotinamide and nicotinic acid. Newcrom AH separates acids using ion-exclusion, retains basic compounds by cation-exchange, and neutral compounds by reverse-phase mechanism.
| Column | Newcrom AH, 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O - 50/50% |
| Buffer | H3PO4 - 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 210 nm |
| Class of Compounds | Acid, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Nicotinic Acid, Nicotinamide, Vitamin B3 |
Application Analytes:
Niacin
Nicotinamide
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)