CAS Number | 98-92-0 |
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Molecular Formula | C6H6N2O |
Molecular Weight | 122.127 g/mol |
InChI Key | DFPAKSUCGFBDDF-UHFFFAOYSA-N |
LogP | -0.370 |
Synonyms |
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A complex mixture of nicotinamide and related impurities was separated on Obelisc R mixed-mode column. Nicotinamide, methylnicotinamide, nicotinamide adenine mononucleotide, nicotinamide adenine dinucleotide, and adenosine monophosphate were baseline resolved in a 15 minute long method. This mixed-mode approach can be used for analysis of other nucleotides. Obelisc R trimodal column separates this complex mixture based on reversed-phase, cation-exchange and anion-exchange mechanisms. Retention is controlled by amount of ACN, buffer concentration and buffer pH. Additional selectivity can be gained by exploring various buffers within the same pH
Column | Newcrom AH, 4.6x150 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O - 50/50% |
Buffer | H3PO4 - 0.5% |
Flow Rate | 1.0 ml/min |
Detection | UV, 210 nm |
Class of Compounds | Acid, Hydrophilic, Ionizable, Vitamin, Supplements |
Analyzing Compounds | Nicotinic Acid, Nicotinamide, Vitamin B3 |