HPLC Separation of Common Antibiotics in Fish Farming
Fish antibiotics are used in fish farming to treat bacterial diseases of fish. It is common practice in the fish industry, particularly in developing countries, to use large amounts of antibiotics to prevent infection. The antibiotics used are often non-biodegradable and remain in the environment for long periods of time, contaminating soil and ground waters. In farming antibiotics are mixed with food and residual amount of drugs ends up in fish products and poultry, this lead to consumption of antibiotics and metabolites by humans. Five common antibiotics are separated on Primesep C column using LC/MS compatible conditions. Method can be used for quantitative determination of nitrofurantoin, nitrofurazone, furazolidone, furaltadone, chloramphenicol and nitrofurazone in fish products and environment.
5-Nitro-2-Furaldehyde Semicarbazone (Nitrofurazone)ChloramphenicolFuraltadoneFurazolidoneNitrofurantoinNitrofurazone
HPLC Separation of Antibiotics in Fish Production
Five antibiotics are separated on Primesep C mixed-mode cation-exchange column using UV, ESLD and LC/MS compatible conditions. Similar compounds can be separated based on reverse phase and cation-exchange mechanisms. Current method can be used in quantitative determination of antibiotics in various food products.
5-Nitro-2-Furaldehyde Semicarbazone (Nitrofurazone)ChloramphenicolFuraltadoneFurazolidoneNitrofurantoin
USP Methods for Nitrofurantoin for the Legacy L1 Column
Application Notes: Nitrofurantoin is an antibiotic used to treat urinary tract infections and E. coli. The USP HPLC method for the separation of nitrofuranton was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of UPS.
Legacy L1 C18 HPLC column
NaH2PO4 pH 7.0/MeCN 88/12
||Legacy L1, 4.6x150 mm, 5 µm, 100A
||: NaH2PO4 pH 7.0/MeCN 88/12
||UV, 254 nm
|Class of Compounds
||Drug, Antibiotics, Hydrophobic, Ionizable
Application USP Methods for Nitrofurantoin for the Legacy L1