Separation of Antibiotics in Mixed-mode chromatography
A complex mixture of sulphonamide, macrolide, tetracycline and fluoroquinolone antibiotics were separated in one run using mixed-mode chromatography with LC/MS -compatible conditions. All compounds are separated based on reversed-phase and/or ion-exchange mechanism. Method can be used for analysis of various classes of antibiotics and related impurities in different sample matrices (blood, urine, soil, waste water).
HPLC Separation of Antibiotics of Group in Mixed-Mode Chromatography
Primesep D was used to separate the antibiotics marbofloxacin, norfloxacin, josamycin, and flumequine. Primesep D is a reverse-phase column with embedded basic ion-pairing groups designed for analysis of plasma. This method is compatible with LC/MS and prep chromatography.
HPLC Retention Control of Antibiotics in Toothpaste
Commercially available toothpaste has been known to include antibiotics. To demonstrate the retention control on the mixed-mode column Primesep 100, toothpaste was spiked with the antibiotics metronidazole and norfloxacin. In a 13 minute method, retention of the antibiotics was controlled by adjustment in ratio of organic in the mobile phase. This method demonstrates the flexibility of using mixed-mode columns such as Primesep 100.