Norfloxacin

CAS Number 70458-96-7
Molecular Formula C16H18FN3O3
Molecular Weight 319.336 g/mol
InChI Key OGJPXUAPXNRGGI-UHFFFAOYSA-N
LogP -1.03
Synonyms
  • Norfloxacin
  • 1-Ethyl-6-fluoro-4-oxo-7-(piperazin-1-yl)-1,4-dihydroquinoline-3-carboxylic acid
  • 3-Quinolinecarboxylic acid, 1-ethyl-6-fluoro-1,4-dihydro-4-oxo-7-(1-piperazinyl)-
  • 70458-96-7
  • 3-Quinolinecarboxylic acid, 1-ethyl-6-fluoro-1,4-dihydro-4-oxo-7-(1-piperazinyl)-
  • 1-Ethyl-6-fluoro-1,4-dihydro-4-oxo-7-(1-piperazinyl)-3-quinolinecarboxylic acid
  • 1-Ethyl-6-fluoro-1,4-dihydro-7-(1-piperazinyl)-4-oxo-3-quinolinecarboxylic acid
  • Amicrobin
  • Baccidal
  • Barazan
  • Chibroxin
  • Chibroxine
  • Chibroxol
  • Esclebin
  • Floxacin
  • Floxacin 400
  • Fulgram
  • Gonorcin
  • Lexinor
  • N-Demethylpefloxacin
  • N-Desmethylpefloxacin
  • Neofloxacin
  • Neofloxin
  • Nolicin
  • Noracin
  • Noraxin
  • Norflox 400
  • Norfloxacine
  • norfloxacino
  • Norfloxin
  • Norocin
  • Noroxin
  • Noroxine
  • Norphloxacine
  • Norxacin
  • Sebercim
  • Spectrama
  • Uroflox
  • Uroxacin
  • Zoroxin
  • BRN 0567897
  • 1,4-Dihydro-1-ethyl-6-fluoro-4-oxo-7-(1-piperazinyl)-3-quinolinecarboxylic acid
  • EINECS 274-614-4
  • Norfloxacinum
  • UNII-N0F8P22L1P
  • 1-Ethyl-6-fluor-1,4-dihydro-4-oxo-7-(1-piperazinyl)-3-chinolincarbonsaeure
  • 1-Ethyl-6-fluoro-1,4-dihydro-4-oxo-7-(1-piperazinyl)-3-quinolinecarboxylic acid
  • NFLX

Applications:


Separation of Antibiotics in Mixed-mode chromatography
  A complex mixture of sulphonamide, macrolide, tetracycline and fluoroquinolone antibiotics were separated in one run using mixed-mode chromatography with LC/MS -compatible conditions. All compounds are separated based on reversed-phase and/or ion-exchange mechanism. Method can be used for analysis of various classes of antibiotics and related impurities in different sample matrices (blood, urine, soil, waste water).  

Condition

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 0-25%, 6 min, 25-70% 14 min
Buffer Gradient Formic Acid - 0.05%-0.3%, 10 min, 14 min hold
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds  Antibiotic, Drug, Hydrophobic, Ionizable
Analyzing Compounds Sulphanilamide, Sulphaguanidine, Oxytetracycline, Marbofloxacin, Sulphadiazine, Chlortetracycline, Nofloxacin, Spiramycin, Cefalonium, Tylosin, Josamycin, Sulphamethoxazole, Dapsone, Sulphaquinoxalline, Flumequine
 

Application Analytes:

Carboxymethylcysteine
Chlortetracycline
Dapsone
Flumequine
Josamycin
Marbofloxacin
Norfloxacin
Oxytetracycline
Spiramycin
Sulfamethoxazole
Sulfonamides
Sulphadiazine
Sulphaguanidine
Sulphanilamide
Sulphaquinoxaline
Tetracycline
Tylosin

Analysis of Antibiotic Norfloxacin and Related Impurities in Mixed-Mode Chromatography
 

Condition 1

Column Primesep D, 4.6x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 5-50%, 10 min, 3 min hold
Buffer H2SO4 - 0.1%
Flow Rate 1.0 ml/min
Detection UV, 250 nm
  The antibiotic norfloxacin is a first generation fluoroquinolone. It is effective against both gram positive and gram negative bacteria. Analysis was done on Obelisc R and Primesep D.  

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 5-30%, 10 min,  5 min hold
Buffer Gradient Formic Acid - 0.05%-0.3%, 10 min,  5 min hold
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds  Antibiotic, Drug, Hydrophobic, Ionizable
Analyzing Compounds  Norfloxacin
 

Application Analytes:

Norfloxacin

HPLC Separation of Antibiotics of Group in Mixed-Mode Chromatography

Primesep D was used to separate the antibiotics marbofloxacin, norfloxacin, josamycin, and flumequine. Primesep D is a reverse-phase column with embedded basic ion-pairing groups designed for analysis of plasma. This method is compatible with LC/MS and prep chromatography.



Application Analytes:

Flumequine
Josamycin
Marbofloxacin
Norfloxacin

HPLC Retention Control of Antibiotics (Metronidazole and Norfloxacine) in Toothpaste
  Commercially available toothpaste has been known to include antibiotics. To demonstrate the retention control on the mixed-mode column Primesep 100, toothpaste was spiked with the antibiotics metronidazole and norfloxacin. In a 13 minute method, retention of the antibiotics was controlled by adjustment in ratio of organic in the mobile phase. This method demonstrates the flexibility of using mixed-mode columns such as Primesep 100.  

Condition

Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H2SO4
Flow Rate 1.0 ml/min
Detection UV, 250 nm
 

Description

Class of Compounds Drug, Antibiotics, Hydrophilic, Ionizable
Analyzing Compounds Metronidazole, Norfloxacine
 

Application Analytes:

Metronidazole
Norfloxacin