CAS Number | 536-21-0 |
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Molecular Formula | C8H11NO2 |
Molecular Weight | 153.181 g/mol |
InChI Key | LRCXRAABFLIVAI-UHFFFAOYSA-N |
LogP | -0.676 |
Synonyms |
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Column | Primesep C, 4.6x150 mm, 5 µm, 100A |
Mobile Phase | MeCN |
Buffer | Formic Acid |
Flow Rate | 1.0 ml/min |
Detection | UV, 250 nm |
Class of Compounds | Drug, Analgetic, Acid, Hydrophilic, Ionizable, |
Analyzing Compounds | Norphenylephrine, Norephedrine, Pseudoephedrine, Doxylamine, Pyrilamine, Dextromethorphan, Trimipramine |
Common hydrophobic basic and hydrophilic basic drugs are separated by mixed-mode chromatography with greater selectivity and resolution than traditional reversed-phase column. Drugs are retained by combination of reversed-phase and cation-exchange mechanisms. Retention time is controlled by amount of acetonitrile, buffer concentration and buffer pH. Available detection techniques are based on buffer selection and include UV, Evaporative Light-Scattering Detector (ELSD), Corona (CAD), LC/MS, etc. This HPLC method can be adopted as general approach for analysis of drugs and pharmaceuticals.
Column | Sharc 1, 3.2x100 mm, 5 µm, 100A |
Mobile Phase | MeCN/MeOH |
Buffer | AmFm, Formic acid |
Flow Rate | 1.0 ml/min |
Detection | UV, 270 nm |
Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements |
Analyzing Compounds | Pseudoephedrine, Norephedrine, Phenylephrine, Norphenylephrine |