CAS Number | 106-49-0 |
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Molecular Formula | C7H9N |
Molecular Weight | 107.156 g/mol |
InChI Key | RZXMPPFPUUCRFN-UHFFFAOYSA-N |
LogP | 1.39 |
Synonyms |
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Toluidine isomers are based on toluene and differ whether the primary amine is ortho, meta or para to the methyl group. A Primesep 200 column with an ammonium formate buffer or a Primesep 100 column with sodium phosphate baseline resolves the three regioisomers. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN), ammonium formate, or sodium phosphate and ultraviolet (UV) detection at 250 nm.
Isomers of toluidine were successfully retained and separated in cation-exchange mode on a Primesep S2 column. Primesep S2 column is a HILIC/cation-exchange column that can be operated in HILIC, cation-exchange, and anion-exclusion modes. Method and column are compatible with ELSD/LC/MS and UV and can be used for routine analysis of polar molecules.
Column | Primesep 200, 4.6x150 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O |
Buffer | AmFm Ph 3.5 |
Flow Rate | 1.0 ml/min |
Detection | UV, 250 nm |
Class of Compounds | Hydrophobic, Ionizable |
Analyzing Compounds | o-Toluidine, 2-Aminobiphenyl, m-Toluidine, p-Toluidine, 4-Aminobiphenyl |