CAS Number | 108-95-2 |
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Molecular Formula | C6H6O |
Molecular Weight | 94.113 g/mol |
InChI Key | ISWSIDIOOBJBQZ-UHFFFAOYSA-N |
LogP | 1.46 |
Synonyms |
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Primesep C demonstrates the control of ion-exchange properties of Primesep columns. The peak order of caffeine and benzylamine in a mixture of caffeine, benzylamine, and phenol reverses when the mobile phase modifier is changed from ammonium acetate to trifluoroacetic acid. Control of the separation is possible even with mass spec (LC/MS) compatible mobile phases of water, acetonitrile (MeCN, ACN) and ammonium acetate or trifluoroacetic acid (TFA).
Column | Primesep 200, Primesep 100 5 µm, 100A |
Mobile Phase | MeCN/H2O |
Buffer | AmFm pH3.0 |
Flow Rate | 0.5 ml/min, 1.0 ml/min |
Detection | UV, 250 nm |
Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Hormone |
Analyzing Compounds | Uracil, Hydroquinone, Phenol, Benzoic Acid, Benzylamine, Lutidine, Toluene, Amitriptyline |
Column | Primesep 200, 3.0x50 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O |
Buffer | AmFm pH3.0 |
Flow Rate | 0.5 ml/min |
Detection | UV, 250 nm |
Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Hormone |
Analyzing Compounds | Uracil, Hydroquinone, Phenol, Benzoic Acid, Benzylamine, Lutidine, Toluene, Amitriptyline |
Column | Obelisc R, 4.6x250 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O - 35/65% |
Buffer | AmAc 10 mM pH 4.0 |
Flow Rate | 1.0 ml/min |
Detection | UV, 250 nm |
Class of Compounds | Drug, Acid, Bases, Neutral, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid |
Analyzing Compounds | Amino acids |
Column | Primesep 200, 4.6*150 mm 5 µm, 100A |
Mobile Phase | MeCN/H2O |
Buffer | H2SO4 |
Flow Rate | 1.0 ml/min |
Detection | UV, 215 nm |
Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Hormone |
Analyzing Compounds | Uracil, Epinephrine, DOPA, 2,6-Lutidine, Benzylamine, Hydroxytrypthophan, Homovanillic acid, Phenol, Tryptophan , 2,3-DHBA, Benzoic acid, Methylparaben, Ethylparaben, Toluene, Amitriptyline |