Phenol

CAS Number 108-95-2
Molecular Formula C6H6O
Molecular Weight 94.113 g/mol
InChI Key ISWSIDIOOBJBQZ-UHFFFAOYSA-N
LogP 1.46
Synonyms
  • Phenol
  • 108-95-2
  • Benzenol
  • CARBOLIC ACID
  • fenol, puro
  • Hydroxy Benzene
  • Hydroxybenzene
  • Monohydroxybenzene
  • Monophenol
  • NSC 36808
  • Oxy Benzene
  • Oxybenzene
  • Phenic Acid
  • Phenyl alcohol
  • Phenyl hydrate
  • Phenyl Hydroxide
  • Phenylic Acid
  • Phenylic alcohol
  • UN 1671
  • UN 2312
  • UN 2821
  • Baker's P & S liquid & Ointment
  • Baker's P and S Liquid and Ointment
  • Caswell No. 649
  • EINECS 203-632-7
  • EPA Pesticide Chemical Code 064001
  • FEMA No. 3223
  • NCI-C50124
  • Phenol, liquefied
  • RCRA waste number U188
  • UNII-339NCG44TV
  • Benzophenol
  • Campho-phenique cold sore gel
  • Campho-phenique gel
  • Campho-phenique liquid
  • Carbolic acid liquid
  • Carbolic oil
  • Carbolicum acidum
  • Carbolsaeure
  • Carbolsaure
  • Cepastat lozenges
  • Cuticura pain relieving ointment
  • Fenol
  • Fenolo
  • Fenosmolin
  • Fenosmoline
  • Hydroxy-benzene
  • IPH
  • IZAL
  • Karbolsaeure
  • Liquefied phenol
  • Liquid phenol
  • Liquified phenol
  • Monohydroxy benzene
  • Monohydroxybenzene
  • Paoscle
  • PhOH
  • Phenic
  • Phenic alcohol
  • Phenol alcohol
  • Phenol homopolymer
  • Phenol liquid
  • Phenol molten
  • Phenol polymer-bound
  • Phenol solution
  • Phenol synthetic
  • Phenolated water
  • Phenolated water for disinfection
  • Phenole
  • Phenosmolin
  • Synthetic phenol
  • Tea polyphenol
  • acide carbolique
  • acide phenique
  • 14534-23-7
  • 50356-25-7
  • 8002-07-1

Applications:


Control of Cation Exchange Properties by pH of Primesep C Column



Primesep C demonstrates the control of ion-exchange properties of Primesep columns. The peak order of caffeine and benzylamine in a mixture of caffeine, benzylamine, and phenol reverses when the mobile phase modifier is changed from ammonium acetate to trifluoroacetic acid. Control of the separation is possible even with mass spec (LC/MS) compatible mobile phases of water, acetonitrile (MeCN, ACN) and ammonium acetate or trifluoroacetic acid (TFA).



Application Analytes:

Benzylamine
Caffeine
Phenol

HPLC Separation of 8 Generic Compounds on Primesep Columns
Mixed-mode HPLC columns allow to analyze compounds with drastically different properties in one run. Acidic, basic, and neutral compounds can be separated in one run using either isocratic or gradient conditions. In this application, neutral hydrophilic (uracil, phenol and hydroquinone), neutral hydrophobic (toluene), hydrophilic acidic (benzoic acid), hydrophilic basic (lutidine) and hydrophobic basic (amitriptyline) are separated using gradient of ACN. Neutral compounds are retained by reversed-phase mechanism, hydrophilic acidic compound become more hydrophobic at lower pH and retain by reversed-phase mechanism too. Basic compounds are retained by cation exchange mechanism, and hydrophobic basic compounds are retained by reversed-phase and cation-exchange mechanisms. All compounds are resolved within 17 minutes on a short column. Method can be applied to various polar and hydrophobic compounds, which can be separated on one column and in one run. Mixed-mode columns can operate in single or combination of several modes: reversed-phase, ion-exchange, ion-exclusion and HILIC. This mixed-mode HPLC column can be used as a general column for separation of wide range of compounds.

Condition

Column Primesep 200, Primesep 100 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm pH3.0
Flow Rate 0.5 ml/min, 1.0 ml/min
Detection UV, 250 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Hormone
Analyzing Compounds Uracil, Hydroquinone, Phenol, Benzoic Acid, Benzylamine, Lutidine, Toluene, Amitriptyline
 

Application Analytes:

Amitriptyline
Benzoic Acid
Benzylamine
Hydroquinone
Lutidine
Phenol
Toluene
Uracil

HPLC Separation of Acidic, Basic, and Neutral Compounds
Primesep 100 and Primesep 200 columns can be used as a universal column for analysis of wide range of compounds. These mixed-mode reversed-phase ion-exchange HPLC columns can provide a valuable alternative to traditional reversed-phase column. Amines, amino acids, quaternary amines, and various zwitter-ions can be analyzed along with hydrophobic compounds and organic and inorganic counter-ions. In this application, 8 compounds with different hydrophobic, hydrophilic, basic and acidic properties are separated based on their properties. Primesep 100 column is a mixed-mode HPLC column with a C12 carbon chain and carboxylic acid on the surface with pKa of 1. Primesep 200 column is a mixed-mode HPLC column with a C12 carbon chain and carboxylic acid on the surface with pKa of 2. These columns can be used with 100% organic (ACN) and 100% aqueous mobile phases. This HPLC method can be adopted as a generic and robust approach for analysis of acidic, basic and neutral compounds within the same run.

Condition

Column Primesep 200, 3.0x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm pH3.0
Flow Rate 0.5 ml/min
Detection UV, 250 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Hormone
Analyzing Compounds Uracil, Hydroquinone, Phenol, Benzoic Acid, Benzylamine, Lutidine, Toluene, Amitriptyline
 

Application Analytes:

Amitriptyline
Benzoic Acid
Benzylamine
Hydroquinone
Lutidine
Phenol
Toluene
Uracil

HPLC Separation of Amino Acids, Bases, Acids, and Neutrals on Obelisc R

Condition

Column Obelisc R, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 35/65%
Buffer AmAc 10 mM pH 4.0
Flow Rate 1.0 ml/min
Detection UV, 250 nm
 

Description

Class of Compounds Drug, Acid, Bases, Neutral, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Amino acids
 

Application Analytes:

2,6-Lutidine
Benzoic Acid
Benzonitrile
Benzylamine
Phenol
Phenylalanine
Pyridine
Toluene
Tryptophan

Generic Screening Method for Complex Mixtures

Condition

Column Primesep 200, 4.6*150 mm 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H2SO4
Flow Rate 1.0 ml/min
Detection UV, 215 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Hormone
Analyzing Compounds Uracil, Epinephrine, DOPA, 2,6-Lutidine, Benzylamine, Hydroxytrypthophan, Homovanillic acid, Phenol, Tryptophan , 2,3-DHBA, Benzoic acid, Methylparaben, Ethylparaben, Toluene, Amitriptyline
 

Application Analytes:

2,3-Dihydroxybenzoic Acid
2,6-Lutidine
Amitriptyline
Benzoic Acid
Benzylamine
DOPA (3,4-dihydroxy-L-phenylalanine)
Epinephrine
Ethyl Paraben
Homovanillic Acid
Hydroxytryptophan
Methylparaben
Phenol
Toluene
Tryptophan
Uracil