|Molecular Weight||166.133 g/mol|
Primesep B combines a hydrophobic, reversed-phase mechanism with ion exchange to separate the diacids, fumaric, benzoic, phthalic, naphthoic, and maleic acids. Changing the acetonitrile content of the mobile phase reverses the peak order for naphthoic and maleic acids. Primesep B combines reversed-phase and anion-exchange mechanism with a mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) and UV detection at 250 nm.
Primesep D separates organic acids such as fumaric, benzoic, phthalic, naphthoic, and maleic acids by a mixture of anion exchange and reversed phase. Retention times and elution order can be changed by adjusting the percentage of acetonitrile in the mobile. This can not be done by traditional ion-exchange and ion-exclusion chromatography. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) and UV detection at 250 nm.
Phthalic acid, phthalic acid isomers, and related products present in the production of phthalic acid were separated on the Primesep D column, based on reversed-phase and in-exchange mechanisms. Neutral, hydrophobic compounds of the phthalic acid production are retained by a reversed-phase mechanism, and phthalic acid and other acidic compounds are retained by a combination of reversed-phase and anion-exchange mechanisms. Resolution and selectivity of this separation can be modified by varying the amount of acetonitrile, buffer concentrations, and buffer pH. This method can be used for monitoring the production cycle of phthalic acid and related impurities.
|Column||Primesep D, 4.6x150 mm, 5 µm, 100A|
|Mobile Phase||Gradient MeCN - 10-50%, 15 min|
|Buffer||H2SO4 - 0.1%|
|Flow Rate||1.0 ml/min|
|Detection||UV, 210 nm|
|Class of Compounds||Acid, Hydrophilic, Ionizable|
|Analyzing Compounds||Terephthalaldehyde, Phthalic acid, 4-Carboxybenzaldehyde, Benzoic acid, Terephthalic acid, p –Tolualdehyde, p-Toluic acid|