Effect of Buffer and Chemistry of Column Stationary Phase on Resolution of Neutral Compounds
In this application, 6 various mixed-mode phases were screened for separation of two neutral compounds. Experiments were conducted on four cation-exchange mixed-mode HPLC columns (Primesep P, Primesep 200, Primesep 100, and Primesep C) and two anion-exchange mixed-mode columns (Primesep D and Primesep B2). All phases showed different selectivity towards HPLC separation of prednisolone and glucose pentaacetate. Application shows that mixed-mode chromatography is a valuable tool in separation of neutral compounds by reversed-phase mechanism. Compounds were monitored by ELSD.
HPLC Separation of Prednisolone, Atrolactic Acid, and Ibuprofen on Mixed-Mode Column
Prednisolone, atrolactic acid, and ibuprofen were separated in one HPLC run on an Obelisc R HPLC column. Obelisc R columns have C12 carbon chain and basic and acidic groups on the surface of silica gel. Compounds are separated by combination of reversed-phase, cation-exchange, and anion-exchange mechanisms. Obelisc R column can be used for retention and separation of acidic and basic hydrophilic compounds, acidic and basic hydrophobic compounds, and neutral compounds. Elution is monitored by common detection techniques like UV, ELSD, CAD and LC/MS.
USP Methods for the Analysis of Prednisolone with the Legacy L1 Column
Application Notes: Prednisolone is a metabolite of prednisone. Prednisone is a common drug used to treat inflammatory diseases. The USP HPLC method for the separation of pyridoxine was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of UPS. Application Columns: Legacy L1 C18 HPLC column
Application compounds: Prednisolone
Mobile phase: MeCN/H20 30/70
Detection technique: UV
Reference: USP35: NF30
HPLC Method of Analysis for Dexamethasone and Prednisolone
View on hplc.cloudDexamethasone is a corticosteroid that is used as a replacement of the natural hormone produced by adrenal glands when the body can’t produce enough on its own. It has also been found to reduce deaths among the critically ill patients with the COVID-19. Prednisolone is another corticosteroid used to treat a variety of inflammatory conditions and has a similar structure to dexamethasone. Both steroids can be retained and separated in HPLC using Newcrom A mixed-mode column with the mobile phase consisting of either acetonitrile (ACN) or methanol (MeOH) and water with formic acid buffer. Methanol offers longer retention time compared to acetonitrile. UV detection at 275nm.