Prednisolone

CAS Number 50-24-8
Molecular Formula C21H28O5
Molecular Weight 360.451 g/mol
InChI Key OIGNJSKKLXVSLS-VWUMJDOOSA-N
LogP 1.62
Synonyms
  • Prednisolone
  • (11beta)-11,17,21-Trihydroxypregna-1,4-diene-3,20-dione
  • Pregna-1,4-diene-3,20-dione, 11,17,21-trihydroxy-, (11beta)-
  • 50-24-8
  • P
  • 4-08-00-03467
  • Pregna-1,4-diene-3,20-dione, 11,17,21-trihydroxy-, (11β)-
  • (11β)-11,17,21-Trihydroxypregna-1,4-diene-3,20-dione
  • 1,2-Dehydrohydrocortisone
  • 1,4-Pregnadiene-11β,17α,21-triol-3,20-dione
  • 1,4-Pregnadiene-3,20-dione-11β,17α,21-triol
  • 11β,17,21-Trihydroxypregna-1,4-diene-3,20-dione
  • 11β,17α,21-Trihydroxypregna-1,4-diene-3,20-dione
  • 1-Dehydrohydrocortisone
  • Codelcortone
  • Co-Hydeltra
  • Cortalone
  • Decaprednil
  • Decortin H
  • Delcortol
  • Delta F
  • Delta-Cortef
  • Deltacortenol
  • Deltacortril
  • Deltacortril Enteric
  • Delta-Ef-Cortelan
  • Deltahydrocortisone
  • Deltasolone
  • Delta-stab
  • Deltisilone
  • Di-Adreson F
  • Dicortol
  • Donisolone
  • Eazolin D
  • Fernisolone
  • Flamasone
  • Hostacortin H
  • Hydeltra
  • Hydeltrone
  • Hydrodeltalone
  • Hydrodeltisone
  • Hydroretrocortin
  • Hydroretrocortine
  • Klismacort
  • Metacortandralone
  • Meticortelone
  • Meti-Derm
  • NSC 9120
  • NSC 9900
  • Panafcortelone
  • Paracortol
  • Precortalon
  • Precortancyl
  • Precortilon
  • Precortisyl
  • Predne-Dome
  • Prednelan
  • Prednicen
  • Predni-Dome
  • Predniretard
  • Prednisolon
  • prednisolona
  • Predonin
  • Predonine
  • Preflam
  • Pregna-1,4-diene-3,20-dione, 11β,17,21-trihydroxy-
  • Prelone
  • Prenolone
  • Scherisolon
  • Sterane
  • Sterolone
  • Supercortisol
  • Ulacort
  • Ultracorten H
  • Ultracortene H
  • Δ1-Cortisol
  • Δ1-Dehydrocortisol
  • Δ1-Dehydrohydrocortisone
  • Δ1-Hydrocortisone
  • BRN 1354103
  • delta(1)-Cortisol
  • Cotogesic
  • Derpo PD
  • Dexa-Cortidelt hostacortin H
  • 3,20-Dioxo-11beta,17alpha,21-trihydroxy-1,4-pregnadiene
  • Dydeltrone
  • EINECS 200-021-7
  • Erbacort
  • Erbasona
  • Estilsona
  • Fernisolone P
  • Lentosone
  • Paracotol
  • Predniliderm
  • Prednis
  • Pregna-1,4-diene-3,20-dione, 11beta,17,21-trihydroxy-
  • 1,4-Pregnadiene-3,20-dione-11-beta,17-alpha,21-triol
  • 1,4-Pregnadiene-11-beta,17-alpha,21-triol-3,20-dione
  • 1,4-Pregnadien-11-beta,17-alpha,21-triol-3,20-dione
  • Rolisone
  • delta(sup 1)-Cortisol
  • delta(sup 1)-Dehydrocortisol
  • delta(sup 1)-Dehydrohydrocortisone
  • delta(sup 1)-Hydrocortisone
  • (11beta)-11,17,21-Trihydroxypregna-1,4-diene-3,20-dione
  • 11beta,17,21-Trihydroxypregna-1,4-diene-3,20-dione
  • 11-beta,17,21-Trihydroxypregna-1,4-diene-3,20-dione
  • Ultracortene-H
  • Fernisolone-P
  • Prednisolonum
  • Bubbli-Pred
  • Cotolone
  • UNII-9PHQ9Y1OLM
  • 1,4-pregnadiene-11beta,17alpha,21-triol-3,20-dione
  • 1,4-pregnadiene-3,20-dione-11beta,17alpha,21-triol
  • 3,20-dioxo-11beta,17alpha,21-trihydroxy-1,4-pregnadiene
  • Delta(1)-dehydrocortisol
  • Delta(1)-dehydrohydrocortisone
  • Delta(1)-hydrocortisone
  • 58201-11-9
  • 8056-11-9

Applications:


Effect of Buffer and Chemistry of Column Stationary Phase on Resolution of Neutral Compounds


In this application, 6 various mixed-mode phases were screened for separation of two neutral compounds. Experiments were conducted on four cation-exchange mixed-mode HPLC columns (Primesep P, Primesep 200, Primesep 100, and Primesep C) and two anion-exchange mixed-mode columns (Primesep D and Primesep B2). All phases showed different selectivity towards HPLC separation of prednisolone and glucose pentaacetate. Application shows that mixed-mode chromatography is a valuable tool in separation of neutral compounds by reversed-phase mechanism. Compounds were monitored by ELSD.



Application Analytes:

D-Glucose Pentaacetate
Prednisolone

Effect of Buffer Concentration on Resolution of Neutral Compounds (Prednisolone and D-Glucose Pentaacetate)


Effect of buffer concentration on retention and selectivity of HPLC separation of prednisolone and glucose pentaacetate was studied on a mixed-mode Primesep P cation-exchange column.



Application Analytes:

D-Glucose Pentaacetate
Prednisolone

HPLC Separation of Prednisolone, Atrolactic Acid, and Ibuprofen on Mixed-Mode Column


Prednisolone, atrolactic acid, and ibuprofen were separated in one HPLC run on an Obelisc R HPLC column. Obelisc R columns have C12 carbon chain and basic and acidic groups on the surface of silica gel. Compounds are separated by combination of reversed-phase, cation-exchange, and anion-exchange mechanisms. Obelisc R column can be used for retention and separation of acidic and basic hydrophilic compounds, acidic and basic hydrophobic compounds, and neutral compounds. Elution is monitored by common detection techniques like UV, ELSD, CAD and LC/MS.



Application Analytes:

Ibuprofen
Prednisolone

USP Methods for the Analysis of Prednisolone with the Legacy L1 Column

 

Application Notes: Prednisolone is a metabolite of prednisone. Prednisone is a common drug used to treat inflammatory diseases. The USP HPLC method for the separation of pyridoxine was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of UPS.  

Application Columns: Legacy L1 C18 HPLC column

Application compounds: Prednisolone

Mobile phase: MeCN/H20 30/70

Detection technique: UV

Reference: USP35: NF30




Application Analytes:

Prednisolone