Pyridine

Pyridine structural formula

CAS Number 110-86-1
Molecular Formula C5H5N
Molecular Weight 79.102 g/mol
InChI Key JUJWROOIHBZHMG-UHFFFAOYSA-N
LogP 0.65
Synonyms
  • Pyridine
  • 110-86-1
  • Azine
  • UN 1282 (DOT)
  • Azabenzene
  • NSC 141574
  • NSC 406123
  • piridina
  • Pyridin
  • UN 1282
  • Caswell No. 717
  • EINECS 203-809-9
  • EPA Pesticide Chemical Code 069202
  • FEMA No. 2966
  • FEMA Number 2966
  • NCI-C55301
  • RCRA waste number U196
  • UNII-NH9L3PP67S
  • Pirydyna
  • Tritisan
  • py
  • 152758-95-7
  • 163392-20-9
  • 45410-39-7
  • 62301-32-0
  • 6999-00-4
  • 733733-47-6
  • 82005-06-9
  • 85404-19-9
  • 85404-20-2
  • 857961-06-9

Applications:


Complex Mixture of Acids, Bases, Amino Acids, and Neutral Compounds
Primesep 100 separates a mixture of amino acids (tyrosine, phenylalanine), organic acids (benzoic acid, mandelic acid), amines (benzylamine, pyridine), and neutrals (benzonitrile, toluene) in one HPLC run by combining reversed-phase, cation-exchange, and polar interactions. The method is tunable and peak order can be changed significantly by adjusting acetonitrile and trifluoroacetic acid concentrations. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) and compatible with UV, mass spec (LC/MS) and evaporative light scattering (ELSD) detection.

Condition

Column Primesep 100, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 30/70%
Buffer TFA - 0.2
Flow Rate 1.0 ml/min
Detection UV, 210 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Tyrosine, phenylalanine, Benzoic acid, mandelic acid, Benzylamine, Pyridine, Benzonitrile, Toluene
 

Application Analytes:

Amino Acids
Benzoic Acid
Benzonitrile
Benzylamine
Mandelic Acid
Organic Acids
Phenylalanine
Pyridine
Toluene
Tyrosine

HPLC Separation of Amino Acids, Bases, Acids, and Neutrals on Obelisc R
Separating basic, acidic and zwitterionic compounds in one run in reverse-phase HPLC can be very challenging. The methods might require the use of ion-pairing reagents and complex gradients that can make MS-compatibility difficult. Obelisc R column which has both positive and negative ion-pairs embedded in the stationary phase allows for fine tuning and separation of a wide range of compounds with different ionic properties. Acids, bases, amino acids and neutral compounds were separated isocratically in one run using a simple MS-compatible mobile phase of acetonitrile (ACN) and water with Ammonium Acetate (AmAc) buffer. Can also be UV detected at 250nm.

Condition

Column Obelisc R, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 35/65%
Buffer AmAc 10 mM pH 4.0
Flow Rate 1.0 ml/min
Detection UV, 250 nm
 

Description

Class of Compounds Drug, Acid, Bases, Neutral, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Amino acids
 

Application Analytes:

2,6-Lutidine
Benzoic Acid
Benzonitrile
Benzylamine
Phenol
Phenylalanine
Pyridine
Toluene
Tryptophan

HPLC Separation of Aminopyridines Isomers in Hydrogen-Bonding mode on a SHARC 1 HPLC Column
  Application Notes: Pyridines and aminopyridines are hydrophilic basic compounds. Traditionally these compounds have been separated and analyzed by GC and HPLC. In the case of HPLC, reversed-phase chromatography with ion-pairing reagent is used along with alternative modes like HILIC for separation. Mixed-mode chromatography can also be used to successfully separate isomers of substituted pyridines. However, we developed a new mode of separation for these compounds with hydrogen bonding. Isomers of aminopyridine are separated based on hydrogen bonding interaction between analyte and stationary phase. Mobile phase utilizes combination of acetonitrile and methanol with additives. Retention time and selectivity are  sensitive to variations of mobile phase.  The order of elution changes depending on the amount of acetonitrile, methanol, formic acid and ammonium formate. This method and approach is compatible with  LC/MS and prep chromatography and can be used for separation of other pyridine based compounds and pyridine based isomers. Application Columns: SHARC 1, 3.2x100 mm, 5 um, 100A, To learn more about SHARC 1 columns click here. To order this column click here. To see more chromatographic separations check our web site. Application Compounds: pyridine, 2-aminopyridine, 3-aminopyridine, 4-aminopyridine Detection Technique: UV, LC/MS  

Condition

Column Sharc 1, 3.2x100 mm, 5 µm, 100A
Mobile Phase MeCN/MeOH
Buffer Fa, AmFm
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug, Muscle strengthener, Hydrophilic, Ionizable, Vertebrate pesticide
Analyzing Compounds 2-Aminopyridine,  3-Aminopyridine, 4-Aminopyridine, Pyridine
 

Application Analytes:

2-Aminopyridine
3-Aminopyridine
4-Aminopyridine
Pyridine

HPLC Separation of Aminopyridines in Non-Aqueous Mobile Phase
 

Condition

Column Sharc 1, 4.6x100 mm, 5 µm, 100A
Mobile Phase MeCN - 97.5%
Buffer H3PO4 - 2.5%
Flow Rate 3.0 ml/min
Detection UV, 210 nm
 

Description

Class of Compounds Drug, Muscle strengthener, Hydrophilic, Ionizable, Vertebrate pesticide
Analyzing Compounds 2-Aminopyridine,  3-Aminopyridine, 4-Aminopyridine, Pyridine
 

Application Analytes:

2-Aminopyridine
3-Aminopyridine
4-Aminopyridine
Pyridine

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