Pyridine

CAS Number 110-86-1
Molecular Formula C5H5N
Molecular Weight 79.102 g/mol
InChI Key JUJWROOIHBZHMG-UHFFFAOYSA-N
LogP 0.65
Synonyms
  • Pyridine
  • 110-86-1
  • Azine
  • UN 1282 (DOT)
  • Azabenzene
  • NSC 141574
  • NSC 406123
  • piridina
  • Pyridin
  • UN 1282
  • Caswell No. 717
  • EINECS 203-809-9
  • EPA Pesticide Chemical Code 069202
  • FEMA No. 2966
  • FEMA Number 2966
  • NCI-C55301
  • RCRA waste number U196
  • UNII-NH9L3PP67S
  • Pirydyna
  • Tritisan
  • py
  • 152758-95-7
  • 163392-20-9
  • 45410-39-7
  • 62301-32-0
  • 6999-00-4
  • 733733-47-6
  • 82005-06-9
  • 85404-19-9
  • 85404-20-2
  • 857961-06-9

Applications:


Complex Mixture of Acids, Bases, Amino Acids, and Neutral Compounds



Primesep 100 separates a mixture of amino acids (tyrosine, phenylalanine), organic acids (benzoic acid, mandelic acid), amines (benzylamine, pyridine), and neutrals (benzonitrile, toluene) in one HPLC run by combining reversed-phase, cation-exchange, and polar interactions. The method is tunable and peak order can be changed significantly by adjusting acetonitrile and trifluoroacetic acid concentrations. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) and compatible with UV, mass spec (LC/MS) and evaporative light scattering (ELSD) detection.



Application Analytes:

Amino Acids
Benzoic Acid
Benzonitrile
Benzylamine
Mandelic Acid
Organic Acids
Phenylalanine
Pyridine
Toluene
Tyrosine

HPLC Separation of Amino Acids, Bases, Acids, and Neutrals on Obelisc R





Application Analytes:

2,6-Lutidine
Benzoic Acid
Benzonitrile
Benzylamine
Phenol
Phenylalanine
Pyridine
Toluene
Tryptophan

HPLC Separation of Aminopyridines Isomers in Hydrogen-Bonding mode on a SHARC 1 HPLC Column

 

Application Notes: Pyridines and aminopyridines are hydrophilic basic compounds. Traditionally these compounds have been separated and analyzed by GC and HPLC. In the case of HPLC, reversed-phase chromatography with ion-pairing reagent is used along with alternative modes like HILIC for separation. Mixed-mode chromatography can also be used to successfully separate isomers of substituted pyridines. However, we developed a new mode of separation for these compounds with hydrogen bonding. Isomers of aminopyridine are separated based on hydrogen bonding interaction between analyte and stationary phase. Mobile phase utilizes combination of acetonitrile and methanol with additives. Retention time and selectivity are  sensitive to variations of mobile phase.  The order of elution changes depending on the amount of acetonitrile, methanol, formic acid and ammonium formate. This method and approach is compatible with  LC/MS and prep chromatography and can be used for separation of other pyridine based compounds and pyridine based isomers.

Application Columns: SHARC 1, 3.2x100 mm, 5 um, 100A, To learn more about SHARC 1 columns click here. To order this column click here. To see more chromatographic separations check our web site.

Application Compounds: pyridine, 2-aminopyridine, 3-aminopyridine, 4-aminopyridine

Detection Technique: UV, LC/MS 

 

 

 




Application Analytes:

2-Aminopyridine
3-Aminopyridine
4-Aminopyridine
Pyridine

HPLC Separation of Aminopyridines in Non-Aqueous Mobile Phase



Application Analytes:

2-Aminopyridine
3-Aminopyridine
4-Aminopyridine
Pyridine