Pyridinedicarboxylic Acid

Pyridinedicarboxylic Acid structural formula

CAS Number499-83-2
Molecular FormulaC7H5NO4
Molecular Weight167.120
InChI KeyWJJMNDUMQPNECX-UHFFFAOYSA-N
LogP0.0746
Synonyms
  • 2,6-Pyridinedicarboxylic acid
  • Pyridine-2,6-dicarboxylic acid
  • 499-83-2
  • 2,6-Dicarboxypyridine
  • 2,6-Dipicolinic acid
  • 6-Carboxypicolinic acid
  • Acide pyridine-2,6-dicarboxylique
  • acido piridina-2,6-dicarboxilico
  • Dipicoline acid
  • Dipicolinic acid
  • NSC 176
  • Pyridin-2,6-dicarbonsaure
  • pyridine-2,6-dicarboxylic acid
  • Dipicolinate
  • EINECS 207-894-3
  • UNII-UE81S5CQ0G

Applications:

HPLC Method for Simultaneous Determination of Nicotinic Acid, Nicotinamide, Pyridine Dicarboxylic Acid, Ethyl Methyl Pyridine and Ethyl Nicotinate on Primesep 100 Column

August 29, 2022

Separation type: Liquid Chromatography Mixed-mode



High Performance Liquid Chromatography (HPLC) Method for Analysis of Nicotinic Acid, Nicotinamide, Pyridine Dicarboxylic Acid, Ethyl Methyl Pyridine and Ethyl Nicotinate Nicotinic acid, also known as Niacin or Vitamin B3, is an essential nutrient that plants and animals synthesize from tryptophan. It is also used as a dietary supplement to treat pellagra. A derivative of Nicotinic acid, Nictotinamide, is a key component of the coenzymes nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP+). Pyridine Dicarboxylic acid (PDA), or Dipicolinic acid (DPA), is a key factor in bacterial endospore heat resistance. It also plays an important role in preparing transition metal complexes for ion chromatography. Ethyl Methyl Pyridine is a common precursor to Nicotinic acid. Ethyl nicotinate, or nicotine acid ethyl ester, is a popular topical treatment for ligament, joint, and muscle pain. These 5 organic compounds can be separated, retained, and analyzed on a Primesep 100 mixed-mode column using an analytical method with a simple gradient mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be UV detected at 250 nm with high resolution and peak symmetry.

Condition

Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN – Gradient
Buffer H2SO4 – 0.05%
Flow Rate 1.0 ml/min
Detection UV 250 nm

 

Description

Class of Compounds Drug, Acid
Analyzing Compounds Nicotinic Acid, Nicotinamide, Pyridine Dicarboxylic Acid, Ethyl Methyl Pyridine and Ethyl Nicotinate

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
2,5-Pyridinedicarboxylic acid
5-Ethyl-2-methylpyridine
Nicotinamide
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)
Pyridinedicarboxylic Acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Pyridinecarboxylic Acids

March 27, 2011


Pyridinecarboxylic acids exist as three isomers with different position of carboxylic acid relative to nitrogen in pyridine. Three isomers of pyridinecarboxylic acid (picolinic or 2-pyridinecarvoxylic acid, niacin or 3-pyridinecarboxylic acid, isonicotinic or 4-pyridinecarboxylic acid), along with pyridinedicarboxylic acid, are separated on a Primesep 100 column. Pyridinecarboxylic acids have a similar empirical formula, and are very similar in terms of hydrophobicity and ionic properties. Small differences in these properties are enough to achieve good separation on cation-exchange mixed-mode HPLC column like Primesep 100. Retention time for all compounds is controlled by the amount of acetonitrile and amount of ions in the mobile phase. Ions in the mobile phase can be created by organic and inorganic acids and corresponding salt buffers. Various detection techniques can be used for monitoring pyridinecarboxylic acids. Other ionizable isomers can be successfully separated on mixed-mode columns.

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Isonicotinic Acid
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)
Organic Acids
Picolinic Acid
Pyridinedicarboxylic Acid

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.