Pyridinedicarboxylic Acid

Pyridinedicarboxylic Acid structural formula

CAS Number 499-83-2
Molecular Formula C7H5NO4
Molecular Weight 167.120 g/mol
LogP 0.0746
  • 2,6-Pyridinedicarboxylic acid
  • Pyridine-2,6-dicarboxylic acid
  • 499-83-2
  • 2,6-Dicarboxypyridine
  • 2,6-Dipicolinic acid
  • 6-Carboxypicolinic acid
  • Acide pyridine-2,6-dicarboxylique
  • acido piridina-2,6-dicarboxilico
  • Dipicoline acid
  • Dipicolinic acid
  • NSC 176
  • Pyridin-2,6-dicarbonsaure
  • pyridine-2,6-dicarboxylic acid
  • Dipicolinate
  • EINECS 207-894-3


HPLC Separation of Pyridinecarboxylic Acids
Pyridinecarboxylic acids exist as three isomers with different position of carboxylic acid relative to nitrogen in pyridine. Three isomers of pyridinecarboxylic acid (picolinic or 2-pyridinecarvoxylic acid, niacin or 3-pyridinecarboxylic acid, isonicotinic or 4-pyridinecarboxylic acid), along with pyridinedicarboxylic acid, are separated on a Primesep 100 column. Pyridinecarboxylic acids have a similar empirical formula, and are very similar in terms of hydrophobicity and ionic properties. Small differences in these properties are enough to achieve good separation on cation-exchange mixed-mode HPLC column like Primesep 100. Retention time for all compounds is controlled by the amount of acetonitrile and amount of ions in the mobile phase. Ions in the mobile phase can be created by organic and inorganic acids and corresponding salt buffers. Various detection techniques can be used for monitoring pyridinecarboxylic acids. Other ionizable isomers can be successfully separated on mixed-mode columns.

Application Analytes:

Isonicotinic Acid
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)
Organic Acids
Picolinic Acid
Pyridinedicarboxylic Acid