Raffinose

CAS Number 512-69-6
Molecular Formula C18H32O16
Molecular Weight 504.438 g/mol
InChI Key MUPFEKGTMRGPLJ-ZQSKZDJDSA-N
LogP -3.34
Synonyms
  • Raffinose
  • beta-D-Fructofuranosyl alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranoside
  • alpha-D-Glucopyranoside, beta-D-fructofuranosyl O-alpha-D-galactopyranosyl-(1->6)-
  • 512-69-6
  • α-D-Glucopyranoside, β-D-fructofuranosylO-α-D-galactopyranosyl-(1-6)-
  • D-(+)-Raffinose
  • D-Raffinose
  • Gossypose
  • Melitose
  • Melitriose
  • NSC 170228
  • NSC 2025
  • rafinosa
  • α-D-Glucopyranoside, β-D-fructofuranosyl O-α-D-galactopyranosyl-(1-6)-
  • EINECS 208-146-9
  • UNII-N5O3QU595M
  • (2R,3R,4S,5R,6R)-2-[(2S,3S,4R,5R)-3,4-dihydroxy-2,5-bis(hydroxymethyl)oxolan-2-yl]oxy-6-[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxymethyl]oxane-3,4,5-triol
  • (2R,3R,4S,5R,6R)-2-[(2S,3S,4R,5R)-3,4-dihydroxy-2,5-bis(hydroxymethyl)tetrahydrofuran-2-yl]oxy-6-[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydropyran-2-yl]oxymethyl]tetrahydropyran-3,4,5-triol(2R,3R,4S,5R,6R)-2-[(2S,3S,4R,5R)-3,4-dihydroxy-2,5-dimethylol-tetrahydrofuran-2-yl]oxy-6-[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-methylol-tetrahydropyran-2-yl]oxymethyl]tetrahydropyran-3,4,5-triol
  • (3R,4S,5R,6R)-2-{[(2R,3S,4S,5R)-6-{[(2S,3S,4S,5R)-3,4-dihydroxy-2,5-bis(hydroxymethyl)oxolan-2-yl]oxy}-3,4,5-trihydroxyoxan-2-yl]methoxy}-6-(hydroxymethyl)oxane-3,4,5-triol
  • 6G-alpha-delta-Galactosylsucrose
  • D-(+)-Raffinose
  • Raffinose hydrate
  • alpha-D-Galp-(1->6)-alpha-D-Glcp-(1<->2)-beta-D-Fruf
  • alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl beta-D-fructofuranoside
  • delta-(+)-Raffinose
  • delta-Raffinose
  • rafinose
  • raflinose
  • 127230-13-1

Applications:


HPLC Separation of Sugars on Primesep S2 Column
Five sugars were separated on a Primesep S2 HILIC column with LC/MS compatible conditions. Various mobile phase produce different selectivity and separation. Method can be used as alternative to aminopropyl column. Primesep S2 column is stable and does not undergo rapid hydrolysis like aminopropyl columns.

Condition

Column Primesep S2, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O -80/20%
Buffer AmAc pH 5.0
Flow Rate 1.0 ml/min
Detection ELSD 50C
 

Description

Class of Compounds Drug, Acid, Monocarboxylic acid,  Hydrophilic, Ionizable, Hormone
Analyzing Compounds Fructose, Glucose, Sucrose, Lactose, Maltose, Sorbitol, Sucrose, Alfa D-Lactose, Beta D–Lactose, Raffinose


Application Analytes:

Fructose
Glucose
Lactose
Maltose
Raffinose
Sorbitol
Sucrose
alpha-D-Lactose
beta-D-Lactose

Separation of Trisaccharides on SIELC Columns
  Trisaccharides can be difficult to separate using conventional HPLC columns. The four trisaccharides panose, raffinose, isomaltotriose, and kestose have the same chemical formula, and the all have two glycosidic bonds connecting the three monosaccharides. Obelisc N was used as a stationary phase to separate trisaccharides because it is capable of multiple modes of separation. Obelisc N is a highly polar column that retains polar and charged analytes. Even though the trisaccharides differ only in regio- and stereochemistry they are resolved.

Condition 

Column Obelisc R, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN - 75%
Buffer AmFm pH 3.0- 10 mM
Flow Rate 1.0 ml/min
Detection ELCD

Description

Class of Compounds Trisaccharides,  Hydrophilic, Ionizable
Analyzing Compounds Raffinose, Kestose, Panose, Isomaltose
 

Application Analytes:

Isomaltotriose
Kestose
Panose
Raffinose