Riboflavin (Vitamin B2)

CAS Number 36791-04-5
Molecular Formula C8H12N4O5
Molecular Weight 244.208 g/mol
InChI Key IWUCXVSUMQZMFG-AFCXAGJDSA-N
LogP -1.85
Synonyms
  • Ribavirin
  • 1-beta-D-Ribofuranosyl-1H-1,2,4-triazole-3-carboxamide
  • 1H-1,2,4-Triazole-3-carboxamide, 1-beta-D-ribofuranosyl-
  • 36791-04-5
  • 1H-1,2,4-Triazole-3-carboxamide, 1-β-D-ribofuranosyl-
  • 1-β-D-Ribofuranosyl-1,2,4-triazol-3-carboxyamide
  • 1-β-D-ribofuranosyl-1,2,4-triazole-3-carboxamide
  • Copegus
  • MegaRibavirin
  • NSC 163039
  • Ravanex
  • Rebetol
  • Ribamide
  • Ribamidil
  • Ribasphere
  • Ribavarin
  • Tribavirin
  • Viramid
  • Virazole
  • Virizadole
  • BRN 0892462
  • Ribamidyl
  • 1-beta-D-Ribofuranosyl-1,2,4-triazole-3-carboxamide
  • Varazid
  • Ribavirin Capsules
  • Ribavirina
  • Ribavirine
  • Ribavirinum
  • UNII-49717AWG6K
  • 1-beta-D-Ribofuranosyl-1H-1,2,4-triazole-3-carboxamide
  • RBV

Applications:


HPLC Separation of Vitamin B
Primesep C separates B vitamins with baseline resolution by a combination of cation exchange, complex formation, and hydrophobic interactions. Vitamin B1 (thiamine), vitamin B2 (riboflavin), and vitamin B6 (pyridoxine) are separated with a mobile phase of water, acetonitrile (MeCN, ACN) and triethylamine (TEA) phosphate with UV detection at 280 nm.

Condition

Column Primesep C , 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O  -10/90%
Buffer NEt3H3PO4 Ph 3.0 - 20 Mm
Flow Rate 1.0 ml/min
Detection UV, 280 nm
 

Description

Class of Compounds Drug, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds Pyridoxine, Thiamine, Riboflavin


Application Analytes:

Pyridoxine
Riboflavin (Vitamin B2)
Thiamine (Vitamin B1)
Vitamin B1 (Thiamine)
Vitamin B2 (Riboflavin)
Vitamins

HPLC Separation of Two Vitamins: Different Polarity - Isocratic Methods
Primesep B and B2 columns separate Vitamins C (ascorbic acid) and B2 (riboflavin) with tunable selectivity. Peak order reversal is exhibited on these two columns with the same mobile phases due to their different polarity. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and either formic or acetic acid (HOAc) with UV detection at 250 nm.

Condition

Column Primesep B, Primesep B2 , 3.2x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Formic Acid, Acetic Acid
Flow Rate 0.5 ml/min
Detection UV, 250 nm
 

Description

Class of Compounds Drug, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds Vitamins C (ascorbic acid) and B2 (riboflavin)


Application Analytes:

Ascorbic Acid
Riboflavin (Vitamin B2)
Vitamin B2 (Riboflavin)
Vitamin C

HPLC Analysis of Active Drug in a Formulation
HPLC method for separation of active ingredients of drug/supplemental composition was developed on an Obelisc R trimodal HPLC column. Compounds are retained by combination of reversed-phase, cation-exchange and anion-exchange mechanisms. Compounds are well separated, and method can be used for quantitation of pyridoxine, ascorbic acid, niacinamide, pantothenic acid, caffeine and riboflavin in a mixture or as separate compounds in various complex mixtures. Various detection techniques can be applied for quantitation (ELSD, UV, LC/MS, Corona). This HPLC method can be adopted as general approach for analysis of active drug components in various formulations.

Condition

Column Obelisc R , 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O  -5/95%
Buffer NaHPO4 pH 3.0 - 30 mM
Flow Rate 1.0 ml/min
Detection UV, 210 nm
 

Description

Class of Compounds Drug, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds Pyridoxine, Ascorbic acid, Niacinamide, Pantothenic acid, Caffeine, Riboflavin


Application Analytes:

Ascorbic Acid
Caffeine
Niacinamide
Pantothenic Acid
Pyridoxine
Riboflavin (Vitamin B2)