Riboflavin (Vitamin B2)

CAS Number 36791-04-5
Molecular Formula C8H12N4O5
Molecular Weight 244.208 g/mol
LogP -1.85
  • Ribavirin
  • 1-beta-D-Ribofuranosyl-1H-1,2,4-triazole-3-carboxamide
  • 1H-1,2,4-Triazole-3-carboxamide, 1-beta-D-ribofuranosyl-
  • 36791-04-5
  • 1H-1,2,4-Triazole-3-carboxamide, 1-β-D-ribofuranosyl-
  • 1-β-D-Ribofuranosyl-1,2,4-triazol-3-carboxyamide
  • 1-β-D-ribofuranosyl-1,2,4-triazole-3-carboxamide
  • Copegus
  • MegaRibavirin
  • NSC 163039
  • Ravanex
  • Rebetol
  • Ribamide
  • Ribamidil
  • Ribasphere
  • Ribavarin
  • Tribavirin
  • Viramid
  • Virazole
  • Virizadole
  • BRN 0892462
  • Ribamidyl
  • 1-beta-D-Ribofuranosyl-1,2,4-triazole-3-carboxamide
  • Varazid
  • Ribavirin Capsules
  • Ribavirina
  • Ribavirine
  • Ribavirinum
  • UNII-49717AWG6K
  • 1-beta-D-Ribofuranosyl-1H-1,2,4-triazole-3-carboxamide
  • RBV


HPLC Separation of Vitamin B
Primesep C separates B vitamins with baseline resolution by a combination of cation exchange, complex formation, and hydrophobic interactions. Vitamin B1 (thiamine), vitamin B2 (riboflavin), and vitamin B6 (pyridoxine) are separated with a mobile phase of water, acetonitrile (MeCN, ACN) and triethylamine (TEA) phosphate with UV detection at 280 nm.

Application Analytes:

Riboflavin (Vitamin B2)
Thiamine (Vitamin B1)
Vitamin B1 (Thiamine)
Vitamin B2 (Riboflavin)

HPLC Separation of Two Vitamins: Different Polarity - Isocratic Methods

Primesep B and B2 columns separate Vitamins C (ascorbic acid) and B2 (riboflavin) with tunable selectivity. Peak order reversal is exhibited on these two columns with the same mobile phases due to their different polarity. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and either formic or acetic acid (HOAc) with UV detection at 250 nm.

Application Analytes:

Ascorbic Acid
Riboflavin (Vitamin B2)
Vitamin B2 (Riboflavin)
Vitamin C

HPLC Analysis of Active Drug in a Formulation

HPLC method for separation of active ingredients of drug/supplemental composition was developed on an Obelisc R trimodal HPLC column. Compounds are retained by combination of reversed-phase, cation-exchange and anion-exchange mechanisms. Compounds are well separated, and method can be used for quantitation of pyridoxine, ascorbic acid, niacinamide, pantothenic acid, caffeine and riboflavin in a mixture or as separate compounds in various complex mixtures. Various detection techniques can be applied for quantitation (ELSD, UV, LC/MS, Corona). This HPLC method can be adopted as general approach for analysis of active drug components in various formulations.

Application Analytes:

Ascorbic Acid
Pantothenic Acid
Riboflavin (Vitamin B2)