Sulphaquinoxaline

Sulphaquinoxaline structural formula

CAS Number59-40-5
Molecular FormulaC14H12N4O2S
Molecular Weight300.340
InChI KeyNHZLNPMOSADWGC-UHFFFAOYSA-N
LogP1.68
Synonyms
  • Sulfaquinoxaline
  • 4-Amino-N-(quinoxalin-2-yl)benzene-1-sulfonamide
  • Benzenesulfonamide, 4-amino-N-2-quinoxalinyl-
  • 59-40-5
  • 5-25-11-00125
  • 2-p-Aminobenzenesulfonamidoquinoxaline
  • 2-p-Aminobenzenesulphonamidoquinoxaline
  • 4-Amino-N-2-quinoxalinylbenzenesulfonamide
  • Avicocid
  • BRN 0290026
  • Caswell No. 721
  • Compound 3-120
  • EINECS 200-423-2
  • EPA Pesticide Chemical Code 077901
  • Italquina
  • Kokozigal
  • NSC 41805
  • N-(2-Quinoxalinyl)sulfanilamide
  • N'-2-Quinoxalylsulfanilamide
  • Sulfabenzpyrazine
  • Sulfacox
  • Sulfaline
  • 2-Sulfanilamidobenzopyrazine
  • 2-Sulfanilamidoquinoxaline
  • 2-(p-Sulfanilamido)quinoxaline
  • Sulphaquinoxaline
  • Sulquin
  • N(sup 1)-(2-Quinoxalinyl)sulfanilamide
  • Ursokoxaline
  • Sulfaquinoxalina
  • Sulfaquinoxalinum
  • UNII-WNW8115TM9
  • Sul-q-nox
  • 8027-68-7

Applications:

HPLC Separation of Antibiotics

June 11, 2020


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Antibiotics are widely used for treatment and prevention of bacterial infections.  Various antibiotics, particularly those with a sulfanilamide structure, were separated in HPLC using mixed-mode columns with varying strengths of ion-pairing groups.  Primesep 200 has weak acidic ion-exchange pairing groups while Newcrom A has strong acidic ion-exchange groups.  In addition, methanol can be used in the mobile phase on Newcrom A column.  The antibiotics were resolved on both columns with a gradient mobile phase consisting of acetonitrile (ACN) or methanol (MeOH), water and sulfuric acid (H2SO4) buffer.  UV detection at 275nm.

Condition 1

Column Primesep 200, 4.6×100 mm, 5 µm, 100A
Mobile Phase MeCN Gradient
Buffer H2SO4 – 0.5%
Flow Rate 1.0 ml/min
Detection UV 275 nm

Condition 2

Column Newcrom A, 3.2×100 mm, 5 µm, 100A
Mobile Phase MeOH Gradient
Buffer H2SO4 Gradient
Flow Rate  0.5 ml/min
Detection UV 275 nm

 

Description

Class of Compounds Drugs, Antibiotics
Analyzing Compounds Sulfanilamide, Sulfadiazine, Sulfaguanidine, Dapsone, Sulfaquinoxaline

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Newcrom A

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Dapsone
Sulfadiazine
Sulfaguanidine
Sulfanilamide
Sulphaquinoxaline
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Antibiotics in Mixed-mode chromatography

May 11, 2015

 

A complex mixture of sulphonamide, macrolide, tetracycline and fluoroquinolone antibiotics were separated in one run using mixed-mode chromatography with LC/MS -compatible conditions. All compounds are separated based on reversed-phase and/or ion-exchange mechanism. Method can be used for analysis of various classes of antibiotics and related impurities in different sample matrices (blood, urine, soil, waste water).

 

Condition

Column Obelisc R, 2.1×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 0-25%, 6 min, 25-70% 14 min
Buffer Gradient Formic Acid – 0.05%-0.3%, 10 min, 14 min hold
Flow Rate 1.0 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
 Antibiotic, Drug, Hydrophobic, Ionizable
Analyzing Compounds Sulphanilamide, Sulphaguanidine, Oxytetracycline, Marbofloxacin, Sulphadiazine, Chlortetracycline, Nofloxacin, Spiramycin, Cefalonium, Tylosin, Josamycin, Sulphamethoxazole, Dapsone, Sulphaquinoxalline, Flumequine

 

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

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Application Analytes:
Carboxymethylcysteine
Chlortetracycline
Dapsone
Flumequine
Josamycin
Marbofloxacin
Norfloxacin
Oxytetracycline
Spiramycin
Sulfamethoxazole
Sulfonamides
Sulphadiazine
Sulphaguanidine
Sulphanilamide
Sulphaquinoxaline
Tetracycline
Tylosin

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.