Tetracyclines are a group of broad spectrum of antibiotics. Structure can be described as derivatives of polycyclic naphthacene carboxamide. Tetracyclines are hydrophilic and basic compounds due to the presence of hydroxyls and amino group. Mixture of tetracyclines and their impurities were separated based on weak reversed-phase and strong cation-exchange mechanisms on a Primesep 200 mixed-mode HPLC column. Elution of compounds was monitored by UV.
Separation of Antibiotics in Mixed-mode chromatography
A complex mixture of sulphonamide, macrolide, tetracycline and fluoroquinolone antibiotics were separated in one run using mixed-mode chromatography with LC/MS -compatible conditions. All compounds are separated based on reversed-phase and/or ion-exchange mechanism. Method can be used for analysis of various classes of antibiotics and related impurities in different sample matrices (blood, urine, soil, waste water).
Obelisc R, 2.1x150 mm, 5 µm, 100A
Gradient MeCN - 0-25%, 6 min, 25-70% 14 min
Gradient Formic Acid - 0.05%-0.3%, 10 min, 14 min hold