Vitamin C | SIELC

CAS Number	50-81-7
Molecular Formula	C6H8O6
Molecular Weight	176.124 g/mol
InChI Key	CIWBSHSKHKDKBQ-JLAZNSOCSA-N
LogP	-1.85
Synonyms	L-Ascorbic acid (5R)-5-[(1S)-1,2-Dihydroxyethyl]-3,4-dihydroxyfuran-2(5H)-one (non-preferred name) 50-81-7 (+)-Ascorbic acid 3-keto-L-Gulofuranolactone 3-Oxo-L-gulofuranolactone Acide ascorbique acido ascorbico Allercorb Antiscorbic vitamin Antiscorbutic vitamin Ascoltin Ascorbajen ASCORBIC ACID, L- Ascorbicap ASCORBINSAEURE Ascorbinsaure Ascorbutina Ascorell Ascorin Ascorteal Ascorvit Cantaxin Catavin C Cebicure Cebion, γ-lactone Cebione Cegiolan Ceglion Celaskon Cemagyl Ce-Mi-Lin Cenetone Cergona Cescorbat Cetamid Cetane-Caps TC Cetemican Cevalin Cevatine Cevimin Ce-Vi-Sol Cevital Cevitamic acid Cevitamin Cevitan Cevitex Chewcee Citrovit Colascor Concemin C-Vimin Davitamon C Juvamine Kangbingfeng L-(+)-Ascorbic acid L-3-Keto-threo-hexuronic acid lactone Laroscorbine Lemascorb Liqui-Cee L-Lyxoascorbic acid L-threo-Ascorbic acid L-threo-Hex-2-enonic acid, γ-lactone L-Xyloascorbic acid Neo-Valdrin NSC 218455 NSC 33832 Planavit C Proscorbin Redoxon Ronotec 100 Rovimix C Rovimix C-EC Scorbu C Secorbate Suncoat VC 40 Testascorbic Vicelat Viforcit Viscorin Viscorin 100M Vitacee Vitacimin Vitacin VITAMIN C Vitamisin Vitascorbol Xyloascorbic acid, L- L-Ascorbate Caswell No. 061B EINECS 200-066-2 FEMA No. 2109 Hex-2-enonic acid gamma-lactone, L-threo- NCI-C54808 L-threo-Hex-2-enonic acid, gamma-lactone Acidum ascorbicum UNII-PQ6CK8PD0R (+)-Sodium L-ascorbate (+)-ascorbate (5R)-5-[(1S)-1,2-dihydroxyethyl]-3,4-dihydroxy-2,5-dihydrofuran-2-one Adenex Allercorb Arco-cee Ascor-B.I.D. Ascorb Ascorbicab Ascorbicin Ascorbin Ascorvit C-Level C-Long C-Quin C-Span Cantan Ce lent Cebid Cebion Cecon Cee-caps TD Cee-vite Ceklin Celaskon Celin Cell C Cemill Cenolate Cereon Cetane Cetane-caps TD Cetebe Cevex Cevi-bid Cevitamate Cewin Ciamin Cipca Citriscorb Dora-C-500 Duoscorb E 300 E-300 E300 Ferrous ascorbate HiCee Hybrin Ido-C Kyselina askorbova L(+)-Ascorbate L(+)-Ascorbic acid L-(+)-Ascorbate L-3-Ketothreohexuronic acid lactone L-Lyxoascorbate L-Xyloascorbate Meri-c Natrascorb Natrascorb injectable Redoxon Ribena Rontex 100 Roscorbic Scorbacid Scorbu-C Sodascorbate VASC Vicin Vicomin C Viscorin 100M Vitace Xitix acidum ascorbinicum gamma-Lactone L-threo-Hex-2-enonate gamma-Lactone L-threo-Hex-2-enonic acid 1018124-03-2 129940-97-2 14536-17-5 154170-90-8 259133-78-3 30208-61-8 50976-75-5 56172-55-5 56533-05-2 57304-74-2 57606-40-3 623158-95-2 882690-91-7 884381-69-5 885512-24-3 88845-26-5 89924-69-6 1428525-25-0 1703051-92-6

Applications:

Separation and Retention of Vitamins in Mixed-Mode HPLC

Primesep B and B2 columns separate Vitamins C (ascorbic acid) and B2 (riboflavin) with tunable selectivity. Both columns allow peak order reversal by simply changing the acetonitrile concentration in the mobile phase. Vitamin B2 is especially sensitive to acetonitrile concentration. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and either trifluoroacetic acid (TFA) or acetic acid (HOAc) with UV detection at 250 nm.

Vitamin B2 (Riboflavin)
Vitamin C

HPLC Separation of Two Vitamins: Different Polarity - Isocratic Methods

Primesep B and B2 columns separate Vitamins C (ascorbic acid) and B2 (riboflavin) with tunable selectivity. Peak order reversal is exhibited on these two columns with the same mobile phases due to their different polarity. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and either formic or acetic acid (HOAc) with UV detection at 250 nm.

Ascorbic Acid
Riboflavin (Vitamin B2)
Vitamin B2 (Riboflavin)
Vitamin C

HPLC Separation of Vitamin C and Vitamin B2

Vitamin C (ascorbic acid) and Vitamin B2 (riboflavin) are water-soluble vitamins that are not stored in the body and must be replaced each day. Primesep D easily resolves Vitamins C and B2 and the elution order can be reversed by adjusting acetonitrile and acetic acid concentrations in the mobile phase. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and acetic acid (AcOH) and UV detection at 250 nm.

Vitamin B2 (Riboflavin)
Vitamin C

Separation of Vitamins in Mixed-Mode HPLC

Separation of Vitamin B2 and Vitamin C is demonstrated on a Primesep D mixed-mode column. This column can be used for analysis of other hydrophobic or neutral vitamins and supplements.

Vitamin B2 (Riboflavin)
Vitamin C

HILIC Separation of Common Preservatives - Citric Acid, Ascorbic Acid and EDTA

Citric acid, ascorbic acid and EDTA are commonly used in food and pharmaceutical industry as preservatives. These compounds are very polar in nature. They are weak organic acids with limited UV activity. Retention and separation is achieved on HILIC mixed-mode Obelisc N column. All three compounds are retained by combination of strong HILIC and strong anion-exchange mechanisms. Separation can be monitored by ELSD, LC/MS, UV or Corona CAD. In contrast to other HILIC column, Obelisc N has two ionizable groups basic and acidic which provide ion-exchange interaction in addition to hydrophilic interaction. This allows to use less acetonitrile for HILIC separation.

Ascorbic Acid
EDTA (Ethylenediaminetetraacetic Acid)
Vitamin C