| CAS Number | 50-81-7 |
|---|---|
| Molecular Formula | C6H8O6 |
| Molecular Weight | 176.124 g/mol |
| InChI Key | CIWBSHSKHKDKBQ-JLAZNSOCSA-N |
| LogP | -1.85 |
| Synonyms |
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Applications:
Separation and Retention of Vitamins in Mixed-Mode HPLC
Primesep B and B2 columns separate Vitamins C (ascorbic acid) and B2 (riboflavin) with tunable selectivity. Both columns allow peak order reversal by simply changing the acetonitrile concentration in the mobile phase. Vitamin B2 is especially sensitive to acetonitrile concentration. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and either trifluoroacetic acid (TFA) or acetic acid (HOAc) with UV detection at 250 nm.
| Column | Primesep B, Primesep B2 , 3.2x50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | Formic Acid, Acetic Acid |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Drug, Vitamin B₆, Hydrophobic, Ionizable |
| Analyzing Compounds | Vitamins C (ascorbic acid) and B2 (riboflavin) |
Application Analytes:
Vitamin B2 (Riboflavin)
Vitamin C
HPLC Separation of Two Vitamins: Different Polarity - Isocratic Methods
Primesep B and B2 columns separate Vitamins C (ascorbic acid) and B2 (riboflavin) with tunable selectivity. Peak order reversal is exhibited on these two columns with the same mobile phases due to their different polarity. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and either formic or acetic acid (HOAc) with UV detection at 250 nm.
| Column | Primesep B, Primesep B2 , 3.2x50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | Formic Acid, Acetic Acid |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Drug, Vitamin B₆, Hydrophobic, Ionizable |
| Analyzing Compounds | Vitamins C (ascorbic acid) and B2 (riboflavin) |
Application Analytes:
Ascorbic Acid
Riboflavin (Vitamin B2)
Vitamin B2 (Riboflavin)
Vitamin C
HPLC Separation of Vitamin C and Vitamin B2
Vitamin C (ascorbic acid) and Vitamin B2 (riboflavin) are water-soluble vitamins that are not stored in the body and must be replaced each day. Primesep D easily resolves Vitamins C and B2 and the elution order can be reversed by adjusting acetonitrile and acetic acid concentrations in the mobile phase. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and acetic acid (AcOH) and UV detection at 250 nm.
| Column | Primesep D , 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | Acetic Acid |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Drug, Vitamin B₆, Hydrophobic, Ionizable |
| Analyzing Compounds | Vitamins C (ascorbic acid) and B2 (riboflavin) |
Application Analytes:
Vitamin B2 (Riboflavin)
Vitamin C
Separation of Vitamins in Mixed-Mode HPLC
Separation of Vitamin B2 and Vitamin C is demonstrated on a Primesep D mixed-mode column. This column can be used for analysis of other hydrophobic or neutral vitamins and supplements.
| Column | Primesep D , 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | Acetic Acid |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Drug, Vitamin B₆, Hydrophobic, Ionizable |
| Analyzing Compounds | Vitamins C (ascorbic acid) and B2 (riboflavin) |
Application Analytes:
Vitamin B2 (Riboflavin)
Vitamin C
HILIC Separation of Common Preservatives - Citric Acid, Ascorbic Acid and EDTA
Citric acid, ascorbic acid, and EDTA are commonly used in food and pharmaceutical industry as preservatives. These compounds are very polar in nature. They are weak organic acids with limited UV activity. Retention and separation is achieved on HILIC mixed-mode Obelisc N column. All three compounds are retained by combination of strong HILIC and strong anion-exchange mechanisms. Separation can be monitored by ELSD, LC/MS, UV or Corona CAD. In contrast to other HILIC column, Obelisc N has two ionizable groups basic and acidic which provide ion-exchange interaction in addition to hydrophilic interaction. This allows to use less acetonitrile for HILIC separation.
| Column | Obelisc N , 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmAc pH 4.0 |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds | Acid, Vitamin B₆, Hydrophobic, Ionizable |
| Analyzing Compounds | Citric acid, ascorbic acid and EDTA |
Application Analytes:
Ascorbic Acid
EDTA (Ethylenediaminetetraacetic Acid)
Vitamin C