Vitamin C

Vitamin C structural formula

CAS Number50-81-7
Molecular FormulaC6H8O6
Molecular Weight176.124
InChI KeyCIWBSHSKHKDKBQ-JLAZNSOCSA-N
LogP-1.85
Synonyms
  • L-Ascorbic acid
  • (5R)-5-[(1S)-1,2-Dihydroxyethyl]-3,4-dihydroxyfuran-2(5H)-one (non-preferred name)
  • 50-81-7
  • (+)-Ascorbic acid
  • 3-keto-L-Gulofuranolactone
  • 3-Oxo-L-gulofuranolactone
  • Acide ascorbique
  • acido ascorbico
  • Allercorb
  • Antiscorbic vitamin
  • Antiscorbutic vitamin
  • Ascoltin
  • Ascorbajen
  • ASCORBIC ACID, L-
  • Ascorbicap
  • ASCORBINSAEURE
  • Ascorbinsaure
  • Ascorbutina
  • Ascorell
  • Ascorin
  • Ascorteal
  • Ascorvit
  • Cantaxin
  • Catavin C
  • Cebicure
  • Cebion, γ-lactone
  • Cebione
  • Cegiolan
  • Ceglion
  • Celaskon
  • Cemagyl
  • Ce-Mi-Lin
  • Cenetone
  • Cergona
  • Cescorbat
  • Cetamid
  • Cetane-Caps TC
  • Cetemican
  • Cevalin
  • Cevatine
  • Cevimin
  • Ce-Vi-Sol
  • Cevital
  • Cevitamic acid
  • Cevitamin
  • Cevitan
  • Cevitex
  • Chewcee
  • Citrovit
  • Colascor
  • Concemin
  • C-Vimin
  • Davitamon C
  • Juvamine
  • Kangbingfeng
  • L-(+)-Ascorbic acid
  • L-3-Keto-threo-hexuronic acid lactone
  • Laroscorbine
  • Lemascorb
  • Liqui-Cee
  • L-Lyxoascorbic acid
  • L-threo-Ascorbic acid
  • L-threo-Hex-2-enonic acid, γ-lactone
  • L-Xyloascorbic acid
  • Neo-Valdrin
  • NSC 218455
  • NSC 33832
  • Planavit C
  • Proscorbin
  • Redoxon
  • Ronotec 100
  • Rovimix C
  • Rovimix C-EC
  • Scorbu C
  • Secorbate
  • Suncoat VC 40
  • Testascorbic
  • Vicelat
  • Viforcit
  • Viscorin
  • Viscorin 100M
  • Vitacee
  • Vitacimin
  • Vitacin
  • VITAMIN C
  • Vitamisin
  • Vitascorbol
  • Xyloascorbic acid, L-
  • L-Ascorbate
  • Caswell No. 061B
  • EINECS 200-066-2
  • FEMA No. 2109
  • Hex-2-enonic acid gamma-lactone, L-threo-
  • NCI-C54808
  • L-threo-Hex-2-enonic acid, gamma-lactone
  • Acidum ascorbicum
  • UNII-PQ6CK8PD0R
  • (+)-Sodium L-ascorbate
  • (+)-ascorbate
  • (5R)-5-[(1S)-1,2-dihydroxyethyl]-3,4-dihydroxy-2,5-dihydrofuran-2-one
  • Adenex
  • Allercorb
  • Arco-cee
  • Ascor-B.I.D.
  • Ascorb
  • Ascorbicab
  • Ascorbicin
  • Ascorbin
  • Ascorvit
  • C-Level
  • C-Long
  • C-Quin
  • C-Span
  • Cantan
  • Ce lent
  • Cebid
  • Cebion
  • Cecon
  • Cee-caps TD
  • Cee-vite
  • Ceklin
  • Celaskon
  • Celin
  • Cell C
  • Cemill
  • Cenolate
  • Cereon
  • Cetane
  • Cetane-caps TD
  • Cetebe
  • Cevex
  • Cevi-bid
  • Cevitamate
  • Cewin
  • Ciamin
  • Cipca
  • Citriscorb
  • Dora-C-500
  • Duoscorb
  • E 300
  • E-300
  • E300
  • Ferrous ascorbate
  • HiCee
  • Hybrin
  • Ido-C
  • Kyselina askorbova
  • L(+)-Ascorbate
  • L(+)-Ascorbic acid
  • L-(+)-Ascorbate
  • L-3-Ketothreohexuronic acid lactone
  • L-Lyxoascorbate
  • L-Xyloascorbate
  • Meri-c
  • Natrascorb
  • Natrascorb injectable
  • Redoxon
  • Ribena
  • Rontex 100
  • Roscorbic
  • Scorbacid
  • Scorbu-C
  • Sodascorbate
  • VASC
  • Vicin
  • Vicomin C
  • Viscorin 100M
  • Vitace
  • Xitix
  • acidum ascorbinicum
  • gamma-Lactone L-threo-Hex-2-enonate
  • gamma-Lactone L-threo-Hex-2-enonic acid
  • 1018124-03-2
  • 129940-97-2
  • 14536-17-5
  • 154170-90-8
  • 259133-78-3
  • 30208-61-8
  • 50976-75-5
  • 56172-55-5
  • 56533-05-2
  • 57304-74-2
  • 57606-40-3
  • 623158-95-2
  • 882690-91-7
  • 884381-69-5
  • 885512-24-3
  • 88845-26-5
  • 89924-69-6
  • 1428525-25-0
  • 1703051-92-6

Applications:

Separation of Vitamins in Mixed-Mode HPLC

August 22, 2010


Separation of Vitamin B2 and Vitamin C is demonstrated on a Primesep D mixed-mode column. This column can be used for analysis of other hydrophobic or neutral vitamins and supplements.

Condition

Column Primesep D , 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Acetic Acid
Flow Rate 1.0 ml/min
Detection UV, 250 nm

 

Description

Class of Compounds
Drug, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds Vitamins C (ascorbic acid) and B2 (riboflavin)

Application Column

Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Vitamin B2 (Riboflavin)
Vitamin C

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HILIC Separation of Common Preservatives – Citric Acid, Ascorbic Acid and EDTA

July 16, 2009

Citric acid, ascorbic acid, and EDTA are commonly used in food and pharmaceutical industry as preservatives. These compounds are very polar in nature. They are weak organic acids with limited UV activity. Retention and separation is achieved on HILIC mixed-mode Obelisc N column. All three compounds are retained by combination of strong HILIC and strong anion-exchange mechanisms. Separation can be monitored by ELSD, LC/MS, UV or Corona CAD. In contrast to other HILIC column, Obelisc N has two ionizable groups basic and acidic which provide ion-exchange interaction in addition to hydrophilic interaction. This allows to use less acetonitrile for HILIC separation.

Condition

Column Obelisc N , 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc pH 4.0
Flow Rate 1.0 ml/min
Detection ELSD

 

Description

Class of Compounds
Acid, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds Citric acid, ascorbic acid and EDTA

Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
Ascorbic Acid
EDTA (Ethylenediaminetetraacetic Acid)
Vitamin C

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Vitamin C and Vitamin B2

November 21, 2006

Vitamin C (ascorbic acid) and Vitamin B2 (riboflavin) are water-soluble vitamins that are not stored in the body and must be replaced each day. Primesep D easily resolves Vitamins C and B2 and the elution order can be reversed by adjusting acetonitrile and acetic acid concentrations in the mobile phase. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and acetic acid (AcOH) and UV detection at 250 nm.

Condition

Column Primesep D , 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Acetic Acid
Flow Rate 1.0 ml/min
Detection UV, 250 nm

 

Description

Class of Compounds
Drug, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds Vitamins C (ascorbic acid) and B2 (riboflavin)

Application Column

Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Vitamin B2 (Riboflavin)
Vitamin C

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation and Retention of Vitamins in Mixed-Mode HPLC

May 5, 2005

Primesep B and B2 columns separate Vitamins C (ascorbic acid) and B2 (riboflavin) with tunable selectivity. Both columns allow peak order reversal by simply changing the acetonitrile concentration in the mobile phase. Vitamin B2 is especially sensitive to acetonitrile concentration. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and either trifluoroacetic acid (TFA) or acetic acid (HOAc) with UV detection at 250 nm.

Condition

Column Primesep B, Primesep B2 , 3.2×50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Formic Acid, Acetic Acid
Flow Rate 0.5 ml/min
Detection UV, 250 nm

 

Description

Class of Compounds
Drug, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds Vitamins C (ascorbic acid) and B2 (riboflavin)

Application Column

Primesep B

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options

Primesep B2

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Vitamin B2 (Riboflavin)
Vitamin C

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Two Vitamins: Different Polarity – Isocratic Methods

May 13, 2004

Primesep B and B2 columns separate Vitamins C (ascorbic acid) and B2 (riboflavin) with tunable selectivity. Peak order reversal is exhibited on these two columns with the same mobile phases due to their different polarity. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and either formic or acetic acid (HOAc) with UV detection at 250 nm.

Condition

Column Primesep B, Primesep B2 , 3.2×50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Formic Acid, Acetic Acid
Flow Rate 0.5 ml/min
Detection UV, 250 nm

 

Description

Class of Compounds
Drug, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds Vitamins C (ascorbic acid) and B2 (riboflavin)

Application Column

Primesep B

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options

Primesep B2

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:

Ascorbic Acid
Vitamin B2 (Riboflavin)
Vitamin C

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.