Vitamin C

CAS Number 50-81-7
Molecular Formula C6H8O6
Molecular Weight 176.124 g/mol
InChI Key CIWBSHSKHKDKBQ-JLAZNSOCSA-N
LogP -1.85
Synonyms
  • L-Ascorbic acid
  • (5R)-5-[(1S)-1,2-Dihydroxyethyl]-3,4-dihydroxyfuran-2(5H)-one (non-preferred name)
  • 50-81-7
  • (+)-Ascorbic acid
  • 3-keto-L-Gulofuranolactone
  • 3-Oxo-L-gulofuranolactone
  • Acide ascorbique
  • acido ascorbico
  • Allercorb
  • Antiscorbic vitamin
  • Antiscorbutic vitamin
  • Ascoltin
  • Ascorbajen
  • ASCORBIC ACID, L-
  • Ascorbicap
  • ASCORBINSAEURE
  • Ascorbinsaure
  • Ascorbutina
  • Ascorell
  • Ascorin
  • Ascorteal
  • Ascorvit
  • Cantaxin
  • Catavin C
  • Cebicure
  • Cebion, γ-lactone
  • Cebione
  • Cegiolan
  • Ceglion
  • Celaskon
  • Cemagyl
  • Ce-Mi-Lin
  • Cenetone
  • Cergona
  • Cescorbat
  • Cetamid
  • Cetane-Caps TC
  • Cetemican
  • Cevalin
  • Cevatine
  • Cevimin
  • Ce-Vi-Sol
  • Cevital
  • Cevitamic acid
  • Cevitamin
  • Cevitan
  • Cevitex
  • Chewcee
  • Citrovit
  • Colascor
  • Concemin
  • C-Vimin
  • Davitamon C
  • Juvamine
  • Kangbingfeng
  • L-(+)-Ascorbic acid
  • L-3-Keto-threo-hexuronic acid lactone
  • Laroscorbine
  • Lemascorb
  • Liqui-Cee
  • L-Lyxoascorbic acid
  • L-threo-Ascorbic acid
  • L-threo-Hex-2-enonic acid, γ-lactone
  • L-Xyloascorbic acid
  • Neo-Valdrin
  • NSC 218455
  • NSC 33832
  • Planavit C
  • Proscorbin
  • Redoxon
  • Ronotec 100
  • Rovimix C
  • Rovimix C-EC
  • Scorbu C
  • Secorbate
  • Suncoat VC 40
  • Testascorbic
  • Vicelat
  • Viforcit
  • Viscorin
  • Viscorin 100M
  • Vitacee
  • Vitacimin
  • Vitacin
  • VITAMIN C
  • Vitamisin
  • Vitascorbol
  • Xyloascorbic acid, L-
  • L-Ascorbate
  • Caswell No. 061B
  • EINECS 200-066-2
  • FEMA No. 2109
  • Hex-2-enonic acid gamma-lactone, L-threo-
  • NCI-C54808
  • L-threo-Hex-2-enonic acid, gamma-lactone
  • Acidum ascorbicum
  • UNII-PQ6CK8PD0R
  • (+)-Sodium L-ascorbate
  • (+)-ascorbate
  • (5R)-5-[(1S)-1,2-dihydroxyethyl]-3,4-dihydroxy-2,5-dihydrofuran-2-one
  • Adenex
  • Allercorb
  • Arco-cee
  • Ascor-B.I.D.
  • Ascorb
  • Ascorbicab
  • Ascorbicin
  • Ascorbin
  • Ascorvit
  • C-Level
  • C-Long
  • C-Quin
  • C-Span
  • Cantan
  • Ce lent
  • Cebid
  • Cebion
  • Cecon
  • Cee-caps TD
  • Cee-vite
  • Ceklin
  • Celaskon
  • Celin
  • Cell C
  • Cemill
  • Cenolate
  • Cereon
  • Cetane
  • Cetane-caps TD
  • Cetebe
  • Cevex
  • Cevi-bid
  • Cevitamate
  • Cewin
  • Ciamin
  • Cipca
  • Citriscorb
  • Dora-C-500
  • Duoscorb
  • E 300
  • E-300
  • E300
  • Ferrous ascorbate
  • HiCee
  • Hybrin
  • Ido-C
  • Kyselina askorbova
  • L(+)-Ascorbate
  • L(+)-Ascorbic acid
  • L-(+)-Ascorbate
  • L-3-Ketothreohexuronic acid lactone
  • L-Lyxoascorbate
  • L-Xyloascorbate
  • Meri-c
  • Natrascorb
  • Natrascorb injectable
  • Redoxon
  • Ribena
  • Rontex 100
  • Roscorbic
  • Scorbacid
  • Scorbu-C
  • Sodascorbate
  • VASC
  • Vicin
  • Vicomin C
  • Viscorin 100M
  • Vitace
  • Xitix
  • acidum ascorbinicum
  • gamma-Lactone L-threo-Hex-2-enonate
  • gamma-Lactone L-threo-Hex-2-enonic acid
  • 1018124-03-2
  • 129940-97-2
  • 14536-17-5
  • 154170-90-8
  • 259133-78-3
  • 30208-61-8
  • 50976-75-5
  • 56172-55-5
  • 56533-05-2
  • 57304-74-2
  • 57606-40-3
  • 623158-95-2
  • 882690-91-7
  • 884381-69-5
  • 885512-24-3
  • 88845-26-5
  • 89924-69-6
  • 1428525-25-0
  • 1703051-92-6

Applications:


Separation and Retention of Vitamins in Mixed-Mode HPLC



Primesep B and B2 columns separate Vitamins C (ascorbic acid) and B2 (riboflavin) with tunable selectivity. Both columns allow peak order reversal by simply changing the acetonitrile concentration in the mobile phase. Vitamin B2 is especially sensitive to acetonitrile concentration. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and either trifluoroacetic acid (TFA) or acetic acid (HOAc) with UV detection at 250 nm.



Application Analytes:

Vitamin B2 (Riboflavin)
Vitamin C

HPLC Separation of Two Vitamins: Different Polarity - Isocratic Methods



Primesep B and B2 columns separate Vitamins C (ascorbic acid) and B2 (riboflavin) with tunable selectivity. Peak order reversal is exhibited on these two columns with the same mobile phases due to their different polarity. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and either formic or acetic acid (HOAc) with UV detection at 250 nm.



Application Analytes:

Ascorbic Acid
Riboflavin (Vitamin B2)
Vitamin B2 (Riboflavin)
Vitamin C

HPLC Separation of Vitamin C and Vitamin B2
Vitamin C (ascorbic acid) and Vitamin B2 (riboflavin) are water-soluble vitamins that are not stored in the body and must be replaced each day. Primesep D easily resolves Vitamins C and B2 and the elution order can be reversed by adjusting acetonitrile and acetic acid concentrations in the mobile phase. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and acetic acid (AcOH) and UV detection at 250 nm.

Application Analytes:

Vitamin B2 (Riboflavin)
Vitamin C

Separation of Vitamins in Mixed-Mode HPLC


Separation of Vitamin B2 and Vitamin C is demonstrated on a Primesep D mixed-mode column. This column can be used for analysis of other hydrophobic or neutral vitamins and supplements.



Application Analytes:

Vitamin B2 (Riboflavin)
Vitamin C

HILIC Separation of Common Preservatives - Citric Acid, Ascorbic Acid and EDTA



Citric acid, ascorbic acid and EDTA are commonly used in food and pharmaceutical industry as preservatives. These compounds are very polar in nature. They are weak organic acids with limited UV activity. Retention and separation is achieved on HILIC mixed-mode Obelisc N column. All three compounds are retained by combination of strong HILIC and strong anion-exchange mechanisms. Separation can be monitored by ELSD, LC/MS, UV or Corona CAD. In contrast to other HILIC column, Obelisc N has two ionizable groups basic and acidic which provide ion-exchange interaction in addition to hydrophilic interaction. This allows to use less acetonitrile for HILIC separation.



Application Analytes:

Ascorbic Acid
EDTA (Ethylenediaminetetraacetic Acid)
Vitamin C