Vitamin E Acetate

CAS Number	58-95-7
Molecular Formula	C31H52O3
Molecular Weight	472.754 g/mol
InChI Key	ZAKOWWREFLAJOT-CEFNRUSXSA-N
LogP	10.3
Synonyms	D-alpha-Tocopheryl acetate (2R)-2,5,7,8-Tetramethyl-2-[(4R,8R)-4,8,12-trimethyltridecyl]-3,4-dihydro-2H-1-benzopyran-6-yl acetate 2H-1-Benzopyran-6-ol, 3,4-dihydro-2,5,7,8-tetramethyl-2-[(4R,8R)-4,8,12-trimethyltridecyl]-, acetate, (2R)- 58-95-7 2H-1-Benzopyran-6-ol, 3,4-dihydro-2,5,7,8-tetramethyl-2-[(4R,8R)-4,8,12-trimethyltridecyl]-, acetate, (2R)- (+)-α-Tocopherol acetate (+)-α-Tocopheryl acetate (2R,4'R,8'R)-α-Tocopherol acetate (2R,4'R,8'R)-α-Tocopheryl acetate (R,R,R)-α-Tocopheryl acetate 2,5,7,8-Tetramethyl-2-(4,8,12-trimethyltridecyl)-6-chromanol acetate 2H-1-Benzopyran-6-ol, 3,4-dihydro-2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-, acetate, [2R-[2R(4R,8R)]]- 2H-1-Benzopyran-6-ol, 3,4-dihydro-2,5,7,8-tetramethyl-2-[(4R,8R)-4,8,12-trimethyltridecyl]-, 6-acetate, (2R)- 6-Chromanol, 2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-, acetate, (+)- Acetate d'α-tocopheryle acetato de α-tocoferilo Combinal E Copherol 12250 Copherol 1250 Covitol Covitol 1100 Covitol 1360 Covitol 700 Covitol 700WD d-Vitamin E acetate D-α-Tocopherol acetate D-α-TOCOPHERYL ACETATE E 230 Clear E-Toplex E-Vicotrat Evipherol Lutavit E 50 Nanotopes Natur-E granulate Spondyvit Tinoderm E TOCOPHEROL ACETATE, (+)-α- TOCOPHERYL ACETATE TOCOPHERYL ACETATE, D-α- TOCOPHERYL D-α ACETATE Tokoferol acetate Vitamin E 230 Clear Vitamin Eα acetate α-Tocopherylacetat Alfacol 2H-1-Benzopyran-6-ol, 3,4-dihydro-2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-, acetate, (2R-(2(4R,8R)))- Contopheron 6-Cromanol, 2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-, acetate, (+)- 3,4-Dihydro-2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-2H-1-benzopyran-6-yl acetate, (2R-(2(4R,8R*)))- Ecofrol EINECS 200-405-4 Ephynal acetate Epsilan-M E-Ferol 2,5,7,8-Tetramethyl-2-(4,8,12-trimethyltridecyl)-6-cromanyl acetate, (+)- Tocopherex (+)-alpha-Tocopherol acetate d-alpha-Tocopherol acetate alpha-Tocopherol acetate, (2R,4'R,8'R)- (+)-alpha-Tocopheryl acetate (R,R,R)-alpha-Tocopheryl acetate (2R,4'R,8'R)-alpha-Tocopheryl acetate Tocophrin Tofaxin Endo E Dompe Vitamin Ealpha acetate Vitamin E acetate, d- UNII-A7E6112E4N Simmyungsaengmosu 12741-00-3 1406-70-8 26243-95-8

Applications:

HPLC Separation of Vitamin E and Vitamin E Acetate on Primesep B Column

Primesep B separates Vitamin E and Vitamin E acetate with reversed peak order of a traditional C8 column. The different selectivity is due to an embedded basic functional group on the stationary phase which contributes polar interactions to the separation. Excellent peak shape results with a mass spec compatible mobile phase of water and acetonitrile (MeCN, ACN) with UV detection at 205 nm.

Condition
Column	Primesep B, 3.2x150 mm, 5 µm, 100A
Mobile Phase	MeCN/H2O - 90/10%
Buffer
Flow Rate	0.5 ml/min
Detection	UV 205

Description
Class of Compounds	Vitamins, Hydrophilic, Ionizable
Analyzing Compounds	Vitamin E, Vitamin E Acetate

Vitamin E
Vitamin E Acetate

HPLC Analysis of ACE Serum Active Ingredients

Mixed-mode HPLC columns allow for separation of compounds with drastically different properties. In this application, highly hydrophilic ascorbic acid is retained and separated from highly hydrophobic retinol and Vitamin E Acetate. Ascorbic acid is retained by anion-exchange mechanism and hydrophobic compounds are retained by reversed-phase mechanism. Compounds are monitored by UV detection. Mobile phase is compatible with LC/MS and method can be used to monitor basic and acidic compounds in bio-fluids (serum, blood, urine, saliva, etc.) retinol and Vitamin E Acetate

Condition
Column	Primesep SB , 3.0x50 mm, 5 µm, 100A
Mobile Phase	MeCN/H2O
Buffer	Acetic Acid
Flow Rate	0.5 ml/min
Detection	UV 270, 325

Description
Class of Compounds	Acid, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds	Ascorbic acid (Vitamin C), Retinol and Vitamin E Acetate

Ascorbic Acid
Vitamin E Acetate