Separation of Antibiotics in Mixed-mode chromatography
A complex mixture of sulphonamide, macrolide, tetracycline and fluoroquinolone antibiotics were separated in one run using mixed-mode chromatography with LC/MS -compatible conditions. All compounds are separated based on reversed-phase and/or ion-exchange mechanism. Method can be used for analysis of various classes of antibiotics and related impurities in different sample matrices (blood, urine, soil, waste water).
Separation of Oxytetracycline and Chlortetracycline in Mixed-Mode
Oxytetracycline and chlortetracycline are polar compounds related to broad-spectrum of tetracycline group of antibiotics. Slight hydrophobicity and presence of the basic group in both compounds allow to employ mixed-mode reversed-phase cation-exchange mechanism for separation of these compounds. Method can be used for analysis of antibiotics and related impurities with LC/MS compatible conditions.