| CAS Number | 68-19-9 |
|---|---|
| Molecular Formula | C63H88CoN14O14P |
| Molecular Weight | 1355.4 |
| InChI Key | FDJOLVPMNUYSCM-WZHZPDAFSA-L |
| Synonyms |
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Applications:
HPLC Method for Separation of 10 Water-Soluble Vitamins on Chromni Column
September 17, 2025
HPLC Method for Separation of 10 Water-Soluble Vitamins on Chromni™ by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for separation of Vitamin B2 (Riboflavin), Nicotinic Acid/Niacin (3-pyridinecarboxylic acid), Nicotinamide, Vitamin B6 (Pyridoxine), Folic Acid, Cyanocobalamin, Thiamine diphosphate (Thiamine pyrophosphate), Vitamin B1 (Thiamine), Pantothenic Acid, Biotin
Riboflavin (B2), Nicotinic Acid (B3), Nicotinamide (B3), Pyridoxine (B6), Folic Acid (B9), Cyanocobalamin (B12), Thiamine Pyrophosphate (coenzyme of B1), Thiamine (B1), Pantothenic Acid (B5), Biotin (B7) are water soluble vitamins with a key function of energy metabolism. These coenzymes are responsible for converting food into usable energy.
Vitamin B2 (Riboflavin), Nicotinic Acid/Niacin (3-pyridinecarboxylic acid), Nicotinamide, Vitamin B6 (Pyridoxine), Folic Acid, Cyanocobalamin, Thiamine diphosphate (Thiamine pyrophosphate), Vitamin B1 (Thiamine), Pantothenic Acid, Biotin can be retained and analyzed using the Chromni™ stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN). Detection is performed using UV.
| Column | Chromni™, 4.6 x 150 mm, 3 µm, 100 A, dual ended |
| Mobile Phase | Gradient of MeCN |
| Buffer | Gradient of Phosphoric Acid |
| Flow Rate | 1.0 ml/min |
| Detection | UV 275 nm, 200 nm |
| Class of Compounds | Vitamins |
| Analyzing Compounds | Vitamin B2 (Riboflavin), Nicotinic Acid/Niacin (3-pyridinecarboxylic acid), Nicotinamide, Vitamin B6 (Pyridoxine), Folic Acid, Cyanocobalamin, Thiamine diphosphate (Thiamine pyrophosphate), Vitamin B1 (Thiamine), Pantothenic Acid, Biotin |
Application Column
Chromni™
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended
Cyanocobalamin
Folic Acid
Nicotinamide
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)
Pantothenic Acid
Thiamine diphosphate (Thiamine pyrophosphate)
Vitamin B1 (Thiamine)
Vitamin B2 (Riboflavin)
Vitamin B6 (Pyridoxine)
HPLC Determination of Cyanocobalamin (Vitamin B-12) on PEI Column
December 21, 2020
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Vitamin B12 is a water-soluble vitamin that is naturally present in some foods and available as a dietary supplement and as prescription medication. Vitamin B12 exists in several forms and contains the mineral cobalt, so compounds with vitamin B12 activity are collectively called “cobalamins”. It plays an essential role in the production of the red blood cells and DNA, as well as in the proper functioning of the nervous system.
Vitamin B12 can be retained and UV detected at 360nm.Using the PEI column and a mobile phase gradient of organic modifier and phosphoric acid (H3PO4) as buffer.
| Column | PEI, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/MeOH |
| Buffer | H3PO4 – 0.4% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 360 nm |
| Class of Compounds |
Vitamin, Drug |
| Analyzing Compounds | Cyanocobalamin (Vitamin B-12) |
Application Column
PEI
SIELC Technologies offers custom phases for customers who require specific separations not achievable with commercially available HPLC phases. Considering the vast array of compounds and mixtures requiring analysis, tailored LC phases can significantly enhance separation results for unique and challenging applications. To learn more about our special custom LC phases designed for your specific separation needs, please contact SIELC Technologies at research@sielc.com. Our team of experts is ready to guide you through the process and create a custom solution that addresses your particular chromatographic challenges.
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