D-Alanine

CAS Number 338-69-2
Molecular Formula C3H7NO2
Molecular Weight 89.094 g/mol
InChI Key QNAYBMKLOCPYGJ-UWTATZPHSA-N
LogP -1.86
Synonyms
  • D-Alanine
  • 338-69-2
  • 2: PN: RU2506269 PAGE: 13 claimed sequence
  • Ba 2776
  • (R)-Alanine
  • ALANINE, D-
  • D-(-)-Alanine
  • D(-)-?-Alanine
  • D-Alanin
  • D-alanina
  • D-?-Alanine
  • NSC 158286
  • EINECS 206-418-1
  • (R)-2-Aminopropionsaeure
  • D-alpha-Aminopropionsaeure
  • UNII-E3UDS4613U
  • (2R)-2-aminopropanoic acid
  • (R)-2-aminopropanoic acid
  • D(-)-a -Alanine
  • D(-)-alpha-Alanine
  • D-Ala
  • D-alpha-alanine
  • D-alpha-aminopropionic acid
  • DAL
  • delta-(-)-Alanine
  • delta-2-Aminopropionic acid
  • delta-Alanine
  • delta-alpha-Alanine
  • D-alanine zwitterion

Applications:


Separation of D-Alanine on Newcrom R1 HPLC column

D-Alanine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Separation of D-Alanine on Newcrom C18 HPLC column
Newcrom R1

This column is available in new type of hardware with only one connection end. Learn more about performance and advantage of this new hardware.



Application Analytes:

D-Alanine

HPLC Separation of Mixture of Non-Essential Amino Acids
 

Condition

Column Primesep 100, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 20/80%
Buffer H3PO4 - 0.1%
Flow Rate 1.0 ml/min
Detection UV, 200 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds L-Aspartic Acid (Asp/D), L-Serine (Ser/S), L-Glutamic Acid Glu/E), L-Alanine Ala/A)
 

Application Analytes:

Amino Acids
Aspartic Acid
D-Alanine
DL-Alanine
GLU (L-Glutamic acid)
Glutamic Acid
Serine

New HPLC Amino Acids Separation Compatible With Carbon Dating Technique
   

Hydroxyproline seems to be the most promising amino acid used in carbon dating when isolated from bone collagen. Separation of amino acids is challenging, especially without the use of ions or inorganic buffers that can interfere with Mass spectrometer (MS) or contaminate the sample with modern carbon. Amino acids are also not retained in reverse-phase chromatography. The ideal solution would be using water only to separate the amino acids. This would allow a direct coupling to MS. We were able to separate hydroxyproline from proline and other simple amino acids like glycine and alanine in HPLC on Newcrom AH column using water only as a mobile phase. Using water also allowed UV detection at 205 nm which can’t be done if using a buffer based on acetic or formic acid. See more information on radiocarbon dating here. The same method can be modified to get symmetrical peaks and higher efficiency if a mobile phase with ionic modifier such as formic acid is used.

Condition

Column Newcrom AH, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Flow Rate 1.0 ml/min
Detection UV, 205 nm, CAD
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Alanine, Glycine, Proline, Hydroxyproline
 

Application Analytes:

Alanine
D-Alanine
Glycine
Hydroxyproline
Proline