| Molecular Formula | C47H55N11O12 |
|---|---|
| Molecular Weight | 966.022 |
| InChI Key | IAOIRKWNLBCFFO-KUDSBEMESA-N |
| Synonyms |
Applications:
HPLC Determination of Darobactin on Primesep Columns
December 23, 2019
HPLC Method for Darobactin on Primesep 200 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Darobactin
The compound was discovered in Photorhabdus bacteria in 2019, living in the digestive systems of entomopathogenic nematodes. Researchers identified the nematode as a possible host because they feed on insects by targeting their larvae and releasing bacteria that then confront pathogens similar to those found in humans.
In experiments, it cured mice of Escherichia coli and Klebsiella pneumoniae infections, without toxic side effects.
| Column | Primesep 200, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Gradient MeCN |
| Buffer | Ammonium Formate pH 3.0 |
| Flow Rate | 1.0 ml/min |
| Detection | UV 280 nm |
| Class of Compounds | Antibiotic |
| Analyzing Compounds | Darobactin |
Application Column
Primesep 200
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
HPLC Determination of Darobactin on Primesep Columns
December 23, 2019
HPLC Method for Darobactin on Primesep SB by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Darobactin
The compound was discovered in Photorhabdus bacteria in 2019, living in the digestive systems of entomopathogenic nematodes. Researchers identified the nematode as a possible host because they feed on insects by targeting their larvae and releasing bacteria that then confront pathogens similar to those found in humans.
In experiments, it cured mice of Escherichia coli and Klebsiella pneumoniae infections, without toxic side effects.
| Column | Primesep SB, 2.1 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Gradient MeCN |
| Buffer | Ammonium Formate pH 3.0 |
| Flow Rate | 0.2 ml/min |
| Detection | UV 280 nm |
| Class of Compounds | Antibiotic |
| Analyzing Compounds | Darobactin |
Application Column
Primesep SB
Column Diameter: 2.1 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
