| CAS Number | 11003-57-9 |
|---|---|
| Molecular Formula | C24H40N5O8 |
| Molecular Weight | 526.29 |
| InChI Key | VEVRNHHLCPGNDU-MUGJNUQGSA-O |
| LogP | -7.26 |
| Synonyms |
Applications:
HPLC Determination of Desmosine on Primesep C Column
January 6, 2021
Desmosine and isodesmosine are unique amino acids found in elastin, a protein in connective tissue such as elastic arteries, lungs, and skin.
Desmosine and isodesmosine can be used as specific biological markers for the degradation of mature cross-linked elastin.
Desmosine can be detected at 270nm using Primesep C mixed-mode reversed-phase cation-exchange column and a mobile phase consisting of acetonitrile (ACN), and water with Ammonium acetate buffer.
| Column | Primesep C, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN – 10% |
| Buffer | Ammonium Acetate pH 5.0 – 20 mM |
| Flow Rate | 1.0 mL/min |
| Detection | UV 270 nm |
| Column | Primesep C, 4.6×150 mm, 5 µm, 100A, |
| Guard | Newcrom R1 4.6×50 mm, 5µm, 100A, |
| Mobile Phase | MeCN – 10% |
| Buffer | Ammonium Acetate pH 5.0 – 20 mM |
| Flow Rate | 1.0 mL/min |
| Detection | UV 270 nm |
| Class of Compounds |
Aminoacid, Poly-amino acid |
| Analyzing Compounds | Desmosine |
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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Primesep C
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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HPLC Method for Analysis of Desmosine
March 16, 2018
Application Column
Obelisc R
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
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