Determination Omeprazole on Obelisc R Column

HPLC.cloud View on hplc.cloud Omeprazole is a proton pump inhibitor, a medication that decreases the amount of acid produced in the stomach.  Omeprazole has a bactericidal effect on Helicobacter pylori. Obelisc R column which has both positive and negative ion-pairs embedded in the stationary phase allows for fine tuning and separation of a wide range of compounds with different ionic properties.  Omeprazole was separated isocratically using a simple MS-compatible mobile phase of acetonitrile (ACN) and water with Ammonium Acetate (AmAc) buffer.  Can also be UV detected at 300nm.

Condition 1

Column Obelisc R, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN -35%
Buffer Ammonium Acetate  pH 5.5 - 10 mM
Flow Rate 1.0 ml/min
Detection UV 300 nm

Condition 2

Column Obelisc R, 4.6x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN -20-50% in 15 min + 5 min hold
Buffer Ammonium Acetate  pH 5.5 - 10 mM
Flow Rate 1.0 ml/min
Detection UV 300 nm
 

Description

Class of Compounds  Drug
Analyzing Compounds Omeprazole
 

Application Analytes:

Omeprazole