Determination Omeprazole on Obelisc R Column


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Omeprazole is a proton pump inhibitor, a medication that decreases the amount of acid produced in the stomach.  Omeprazole has a bactericidal effect on Helicobacter pylori.

Obelisc R column which has both positive and negative ion-pairs embedded in the stationary phase allows for fine tuning and separation of a wide range of compounds with different ionic properties.  Omeprazole was separated isocratically using a simple MS-compatible mobile phase of acetonitrile (ACN) and water with Ammonium Acetate (AmAc) buffer.  Can also be UV detected at 300nm.

Condition 1

Column Obelisc R, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN -35%
Buffer Ammonium Acetate  pH 5.5 – 10 mM
Flow Rate 1.0 ml/min
Detection UV 300 nm

Condition 2

Column Obelisc R, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN -20-50% in 15 min + 5 min hold
Buffer Ammonium Acetate  pH 5.5 – 10 mM
Flow Rate 1.0 ml/min
Detection UV 300 nm

 

Description

Class of Compounds
 Drug
Analyzing Compounds Omeprazole

 

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

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Application Analytes:
Omeprazole
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.