HPLC Method for Analysis of Pesticides: Isoproturon, Diuron, Acenaphthene, Carbendazim
Isoproturon is an agricultural herbicide with a tendency to adsorb to soils, which leads to it entering bodies of water. In that water, it is highly toxic for algae and oysters, while long term exposure to the pesticide affects the growth of fish. Diuron is a pre-emergent that is used for non-crop areas as well as with many agricultural crops. It has a low acute toxicity to mammals. It can cause irritation of nose and throat.if inhaled and irritation of eyes if comes in contact with the eyes. Acenaphthene is derived from coal tar and has many used such as dyes, pharmaceuticals, insecticides, fungicides, and plastics. It is harmful if ingested, inhaled or comes in contact with skin, and can cause irritations to skin, eyes, upper respiratory tract, and mucous membranes Carbendazim, also known as mercazole, is a broad-spectrum benzimidazole fungicide. It’s used mostly as a worm control agent in places like tennis courts and golf greens. Sometimes it is also used to control plant diseases in cereals and fruits. Primesep 100, a reverse-phase column, contains embedded acidic ionizable groups and can retain Isoproturon, Diuron, Acenaphthene, and Carbendazim. The method is UV compatible and can be used as a general approach for analyzing similar compounds.
Diuron can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
This column is available in new type of hardware with only one connection end.
Learn more about performance and advantage of this new hardware.
HPLC Separation of Pesticides, Herbicides, Fungicides and Insecticides on Newcrom BH Column
Herbicides are used to control unwanted plants, they are also known as weedkillers. Insecticides are used to kill insects. Fungicides are used to kill parasitic fungi. Pesticide is a more generic term that includes herbicides, fungicides and insecticides in its definition. All are heavily used in agriculture. By using HPLC, many different pesticides can be separated and their retention characteristics controlled using the Newcrom BH mixed-mode column. It can be used with different organic media such as acetonitrile (ACN) or methanol (MeOH). By varying the concentration of organic modifier and using different buffers like sulfuric acid (H2SO4), ammonium formate (AmFm), or formic acid, separation of desired pesticides can be achieved.