CAS Number | 443-79-8 |
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Molecular Formula | C6H13NO2 |
Molecular Weight | 131.176 g/mol |
InChI Key | AGPKZVBTJJNPAG-UHFFFAOYSA-N |
LogP | -0.720 |
Synonyms |
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DL-Isoleucine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
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Column | Primesep 100, 4.6x250 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O - 25/75% |
Buffer | Gradient H2SO4 0.05-0.2% 25 min |
Flow Rate | 1.0 ml/min |
Detection | UV, 200 nm |
Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid |
Analyzing Compounds | L-Threonine (Thr/T), L-Valine (Val/V), DL-Methionine (Met/M), L-Isoleucine (Ile/I), L-Leucine (Leu/L), L-Phenylalanine (Phe/F), L-Histidine (His/H), L- Tryptophan (Trp/W) |
Column | Primesep 100, 4.6x250 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O - 35/65% |
Buffer | H2SO4 0.05% 12 min hold, gradient 0.05-0.20, 13 min |
Flow Rate | 1.0 ml/min |
Detection | UV, 200 nm |
Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid |
Analyzing Compounds | L-Asparagine (Asn/N), L-Threonine (Thr/T), L-Cysteine (Cys/C), L-Proline (Pro/P), L-Valine (Val/V), DL-Methionine (Met/M), L-Isoleucine (Ile/I), L-Leucine (Leu/L), L-Phenylalanine (Phe/F), L- Tryptophan (Trp/W), L-Histidine (His/H), L-Arginine (Arg/R) |
Column | Primesep 100, 4.6x250 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O - 5/95% |
Buffer | AmFM pH 3.0 - 30 mM |
Flow Rate | 1.0 ml/min |
Detection | CAD (Corona) MS- compatible mobile phase |
Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid |
Analyzing Compounds | Allo-Isoleucine, Isoleucine, Leucine |