Doxycycline

Doxycycline structural formula

CAS Number564-25-0
Molecular FormulaC22H24N2O8
Molecular Weight444.441
InChI KeyJBIWCJUYHHGXTC-AKNGSSGZSA-N
LogP-0.0200
Synonyms
  • Doxycycline
  • (4S,4aR,5S,5aR,6R,12aS)-4-(Dimethylamino)-3,5,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-1,4,4a,5,5a,6,11,12a-octahydrotetracene-2-carboxamide
  • 2-Naphthacenecarboxamide, 4-(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,5,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-, (4S,4aR,5S,5aR,6R,12aS)-
  • 564-25-0
  • DOX
  • DOXY
  • 2-Naphthacenecarboxamide, 4-(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,5,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-, (4S,4aR,5S,5aR,6R,12aS)-
  • 2-Naphthacenecarboxamide, 4-(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,5,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-
  • 2-Naphthacenecarboxamide, 4-(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,5,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-, [4S-(4?,4a?,5?,5a?,6?,12a?)]-
  • 4-(Dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,5,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-2-naphthacenecarboxamide
  • 5-Hydroxy-?-6-deoxytetracycline
  • 6-Deoxy-5-hydroxytetracycline
  • 6-Deoxyoxytetracycline
  • Dentistar
  • Deoxycycline
  • Deoxymykoin
  • doxiciclina
  • Doximal
  • Doxinyl
  • Doxivetin
  • Doxycen
  • Doxycyclin
  • Doxytetracycline
  • Hydramycin
  • Liviatin
  • Medeomycin
  • Monodox
  • Oxytetracycline, 6-deoxy-
  • Pulmodox
  • Ronaxan
  • Tolexine
  • Vibramycin
  • Vibramycine
  • Vibranos SF
  • Vibraveineuse
  • Vibravenos
  • ?-6-Deoxy-5-hydroxytetracycline
  • ?-6-Deoxyoxytetracycline
  • ?-Doxycycline
  • Azudoxat
  • alpha-6-Deoxy-5-hydroxytetracycline
  • alpha-6-Deoxyoxytetracycline
  • 6-alpha-Deoxy-5-oxytetracycline
  • 6-Deoxytetracycline
  • Doxitard
  • Doxy-Caps
  • Doxy-Puren
  • Doxy-Tabs
  • EINECS 209-271-1
  • 5-Hydroxy-alpha-6-deoxytetracycline
  • Investin
  • alpha-Doxycycline
  • Doxychel
  • Dossiciclina
  • Doxycyclinum
  • UNII-334895S862
  • Doxysol
  • 6alpha-deoxy-5-oxytetracycline
  • doxycycline (anhydrous)

Applications:

HPLC Method For Analysis Of Ivermectin and Doxycycline on Primesep 100 Column

August 31, 2021

HPLC Method for Ivermectin B1a, Doxycycline, Ivermectin B1b on Primesep 100 by SIELC Technologies

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Ivermectin and Doxycycline

Ivermectin is an anti-parasitic drug. 1A variation has the chemical formula C48H74O14. 1b has the chemical formula C47H72O14. It is often used to treat or prevent parasites in animals and humans. In dogs, it is routinely used to treat heartworm, though, certain breeds, can be severely poisoned by this medication. You can find detailed UV spectra of Ivermectin B1a and information about its various lambda maxima by visiting the following link.

Doxycycline is a broad spectrum antibiotic with the chemical formula C22H24N2O8. Outside of being an antibiotic, it is also said o have anti-inflammatory and anti-angiogenic properties. It is used primarily to treat Lyme disease. chronic prostatitis, sinusitis, pelvic inflammatory disease, acne, rosacea, and rickettsia. You can find detailed UV spectra of Doxycycline  and information about its various lambda maxima by visiting the following link.

Ivermectin and Doxycycline can be retained and separated on the Primesep 100 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and sulphuric acid (H2SO4) buffer. The analysis method can be UV detected at 250 nm.

Condition
Column Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 65/35%
Buffer H2SO4
Flow Rate 1.0 ml/min
Detection UV, 250 nm

 

Description
Class of Compounds
 Drug, Antibiotics
Analyzing Compounds Ivermectin B1a, Doxycycline, Ivermectin B1b

 

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Doxycycline
Ivermectin B1a
Ivermectin B1b
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Doxycycline on Newcrom R1 HPLC column

February 16, 2018
Separation of Doxycycline on Newcrom C18 HPLC column

Doxycycline can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Doxycycline
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.