Drotaverine

 

 

CAS Number14009-24-6
Molecular Formula C24H31NO4
Molecular Weight397.5
InChI KeyOMFNSKIUKYOYRG-MOSHPQCFSA-N
LogP5.4
Synonyms
  • dihydroisoperparine
  • drotaverin
  • drotaverin hydrochloride
  • drotaverine
  • isodihydroperparine
  • No-shpa
  • No-spa
  • Nospan
  • Nospanum

Applications:

HPLC Method For Analysis of Drotaverine (No-spa) on Primesep B Column

September 27, 2021

HPLC Method for Drotaverine on Primesep B by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Drotaverine.

Drotaverine (known under the brand name No-Spa in Europe) is an antispasmodic commonly encountered in the hospital setting to increase cervical dilation during childbirth. It is also commonly used to alleviate gastrointestinal and genitourinary smooth muscle spasms. There have been issues with the drug being counterfeited or smuggled into countries that have yet to approve it, so having an HPLC method to detect the drug and seperate it from impurities and other unwanted compounds is a must for the pharmaceutical community. It’s chemical formula is C24H31NO4.

Drotaverine can be detected in the high UV regime. Using a Primesep B reverse-phase column and a mobile phase consisting of water and an acetonitrile (MeCN) gradient with a sulfuric acid (H2SO4) buffer, drotaverine can be retained, separated (from impurities and other unwanted compounds). This analysis method can be UV detected at 360 nm.

Condition
Column Primesep B, 3.2 x 100 mm, 5 µm, 100 A, dual ended
Mobile Phase Gradient MeCN – 10-50%, 10 min
Buffer H2SO4 – 0.1%
Flow Rate 0.5 ml/min
Detection UV 245, 305, 360  nm

Description
Class of Compounds Drug
Analyzing Compounds Drotaverine

 

Application Column

Primesep B

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Drotaverine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.