CAS Number 75847-73-3
Molecular Formula C20H28N2O5
Molecular Weight 376.454 g/mol
LogP 0.07
  • Enalapril
  • N-[(2S)-1-Ethoxy-1-oxo-4-phenylbutan-2-yl]-L-alanyl-L-proline
  • L-Proline, N-[(1S)-1-(ethoxycarbonyl)-3-phenylpropyl]-L-alanyl-
  • 75847-73-3
  • L-Proline, N-[(1S)-1-(ethoxycarbonyl)-3-phenylpropyl]-L-alanyl-
  • (S)-1-(N-(1-(Ethoxycarbonyl)-3-phenylpropyl)-L-alanyl)-L-proline
  • Bonuten
  • Enalapril Richet
  • Gadopril
  • Enalaprila
  • Enalaprilum
  • UNII-69PN84IO1A
  • 1-(N-((S)-1-carboxy-3-phenylpropyl)-L-alanyl)-L-proline 1'-ethyl ester


Separation of Enalapril on Newcrom R1 HPLC column

Enalapril is a drug used to treat high blood pressure, diabetic kidney disease, and heart failure. It works by inhibiting angiotensin II, a blood pressure raiser. Enalapril can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particle columns are available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation of impurities in preparative separation. It also suitable for pharmacokinetics.

Separation of Enalapril on Newcrom C18 HPLC column
Newcrom R1

This column is available in new type of hardware with only one connection end. Learn more about performance and advantage of this new hardware.

Application Analytes:


HPLC Separation of Enalapril and Lisinopril on Newcrom AH Column
Separation type: Liquid Chromatography Mixed-mode

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High Performance Liquid Chromatography (HPLC) Method for Analysis of Enalapril and Lisinopril
Enalapril and lisinopril are medications that work on the kidneys to help lower blood pressure. Lisinopril can be used for heart failure and for people who’ve had a heart attack. Enalapril can also be used for heart failure but not heart attacks. By using a Newcrom AH mixed-mode column with a cation-exchange mechanism, enalapril and lisinopril, can be baseline separated in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN) and a sulfuric acid (H2SO4) buffer. UV detection at 210 nm.


Column Newcrom AH, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 50/50%
Buffer  H2SO4 - 0.5%
Flow Rate 1.0 ml/min
Detection UV, 210 nm


Class of Compounds Drug
Analyzing Compounds Enalapril,  Lisinopril, Maleic Acid

Application Analytes:

Lisinopril dihydrate