Erythrosine

Molecular FormulaC20H6I4Na2O5
Molecular Weight879.9
InChI KeyRAGZEDHHTPQLAI-UHFFFAOYSA-L
Synonyms
  • 16423-68-0
  • Erythrosin B
  • Erythrosine B
  • ERYTHROSINE
  • C.I. Acid Red 51
  • Erythrosine Lake
  • Erythrosin extra bluish
  • Sodium 2',4',5',7'-tetraiodo-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-bis(olate)
  • Tetraiodofluorescein sodium salt
  • Food Red No. 3
  • C.I. Food Red 14
  • FD and C Red No. 3
  • Erythrosine I
  • Erythrosine BS
  • Erythrosine TB
  • Erythrosine 3B
  • Maple erythrosine
  • Sodium erythrosin
  • Erythrosine Extra
  • Cilefa Pink B
  • Erythrosine Bluish
  • Erythrosine B Disodium Salt
  • Dolkwal erythrosine
  • Erythrosin B, pure, certified
  • Food Dye Red 3
  • Erythrosine TB Extra
  • FDC Red 3 dye
  • Food Red 14
  • Usacert Red No. 3
  • Calcocid erythrosine N
  • Schultz No. 887
  • Hexacert Red No. 3
  • Hexacol erythrosine BS
  • FD and C Red 3
  • New Pink Bluish Geigy
  • Red Dye No. 3
  • Canacert erythrosine BS
  • Erythrosine (indicator)
  • Caswell No. 425AB
  • FDC Red 3
  • Erythrosine Extra Bluish
  • LB-Rot 1
  • Erythrosine Extra Pure A
  • D&C Red No. 3
  • Edicol Supra Erythrosin AS
  • Edicol Supra Erythrosine A
  • disodium;2',4',5',7'-tetraiodo-3-oxospiro[2-benzofuran-1,9'-xanthene]-3',6'-diolate
  • Cerven kysela 51 [Czech]
  • Cerven kysela 51
  • Sodium 2',4',5',7'-tetraiodo-3-oxo-3H-spiro-[isobenzofuran-1,9'-xanthene]-3',6'-bis(olate)
  • CCRIS 892
  • Dye FD and C Red No. 3
  • Erythrosine B (biological stain)
  • Cerven potravinarska 14 [Czech]
  • Erythrosine Extra Conc. A Export
  • Cerven potravinarska 14
  • EINECS 240-474-8
  • Erythrosine B-FO (biological stain)
  • Erythrosine K-FO (biological stain)
  • Erythrosine bluish (biological stain)
  • EPA Pesticide Chemical Code 120901
  • Erythrosine sodium (close form) [USAN]
  • C20H6I4Na2O5
  • AI3-09094
  • Disodium 2',4',5',7'-tetraiodofluorescein
  • E 127
  • HSDB 7974
  • 2',4',5',7'-Tetraiodofluorescein, disodium salt
  • Erythrosin B disodium
  • Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, 3',6'-dihydroxy-2',4',5',7'-tetraiodo-, disodium salt
  • CI 45430
  • Disodium 2-(2,4,5,7-tetraiodo-6-oxido-3-oxoxanthen-9-yl)benzoate
  • 9-(o-Carboxyphenyl)-6-hydroxy-2,4,5,7-tetraiodo-3H-xanthene-3-one disodium salt monohydrate
  • Disodium 9-(O-carboxyphenyl)-6-hydroxy-2,4,5,7-tetraiodo-3H-xanthen-3-one monohydrate
  • SCHEMBL19080
  • Disodium 3',6'-dihydroxy-2',4',5',7'-tetraiodospiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one
  • CHEMBL2106219
  • DTXSID7021233
  • Erythrosin B, analytical standard
  • BDBM50523756
  • AKOS015904033
  • AKOS016010498
  • NSC 759227
  • 3',6'-Dihydroxy-2',4',5',7'-tetraiodospiro(isobenzofuran-1(3H),9'- -(9H)xanthen)-3-one, disodium salt
  • Erythrosin extra bluish (C.I. 45430)
  • M376
  • Erythrosin extra bluish, 87.0-100.0%
  • FT-0625707

Applications:

HPLC Method For Analysis of Erythrosine on Newcrom BH Column

September 13, 2021

Separation type: Liquid Chromatography Mixed-mode


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High Performance Liquid Chromatography (HPLC) Method for Analysis of Erythrosine

Erythrosine (aka Red #3) is a fluorescent red acid dye used for food coloring, pharmaceutical dye, in printing inks, as a biological stain, and as a dental plaque disclosing agent. Erythrosine can be retained on HPLC mixed-mode Newcrom BH column using a mobile phase consisting of methanol (MeOH) and water with perchloric acid (HClO4) buffer. The analysis method can be UV detected at 240 or 498 nm.

Condition
Column Newcrom BH, 2.1×100 mm, 5 µm, 100A
Mobile Phase MeOH/H2O – 75/25%
Buffer HClO4 – 0.5%
Flow Rate 0.2 ml/min
Detection UV 240, 480  nm

Description
Class of Compounds Acid, Dyes
Analyzing Compounds Erythrosine FD and C Red No. 3

 

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Erythrosine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.