|Molecular Weight||138.060 g/mol|
Fosfomycin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
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Fosfomycin is a broad-spectrum antibiotic consisting of an epoxide ring and a phosphite group. It can be retained on a Primesep SB column in about 8 minutes using an isocratic MS-compatible mobile phase method. Primesep SB is a reverse-phase (RP) mixed-mode column with embedded strong basic ion-pairing groups.
|Column||Primesep SB, 3.2x150 mm, 5 µm, 100A|
|Mobile Phase||MeCN/H2O - 20/80%|
|Buffer||AmFm pH 3.0- 20 mM|
|Flow Rate||0.5 ml/min|
|Detection||CAD (Corona) MS- compatible mobile phase|
|Class of Compounds||Drug, Acid, Hydrophilic, Ionizable, Antibiotic|