HPLC Determination of Coenzyme Q10 and Ubiquinol on Primesep D Column

Coenzyme Q10, aka ubiquinone, is an enzyme found in animals and is used as a dietary supplement. Ubiquinol is a reduced form of coenzyme Q10. The two compounds are similar in structure and can be difficult to separate in reverse-phase HPLC. Using mixed-mode Primesep D column and the mobile phase with acetonitrile (ACN) and water, without the need for buffer, CoQ10 and ubiquinol can be isocratically separated and UV detected at 275nm.


Column Primesep D
Mobile Phase MeCN/H2O - 95/5%
Buffer No
Flow Rate 1.0 ml/min
Detection UV 275 nm, Vis 435 nm


Class of Compounds Hydrophobic
Analyzing Compounds Coenzyme Q10,  Ubiquinol

Application Analytes:

Coenzyme q10