HPLC Determination of Darobactin on Primesep Columns

HPLC Determination of Darobactin on Primesep 200 Column_1212

HPLC Determination of Darobactin on Primesep SB Column_1213
The compound was discovered in Photorhabdus bacteria in 2019, living in the digestive systems of entomopathogenic nematodes. Researchers identified the nematode as a possible host because they feed on insects by targeting their larvae and releasing bacteria that then confront pathogens similar to those found in humans.

In experiments, it cured mice of Escherichia coli and Klebsiella pneumoniae infections, without toxic side effects.

Condition

Column Primesep 200, Primesep SB
Mobile Phase Gradient  MeCN
Buffer Ammonium Formate pH 3.0
Flow Rate 1.0 ml/min
Detection UV 280 nm

Description

Class of Compounds Antibiotic
Analyzing Compounds Darobactin

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options

Primesep SB

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Darobactin
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.