HPLC Method for Analysis of Divinyl sulfone on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Divinyl Sulfone
Divinyl sulfone (C4H6O2S) is an organic compound with a vinyl group (-CH=CH2) attached to a sulfone group (-SO2-). It’s a colorless liquid that is used in organic synthesis and polymer chemistry due to its ability to act as a crosslinker.
Applications:
- In bioconjugation chemistry, it’s used for modifying proteins or attaching drugs to carriers.
- It’s used to prepare hydrogels for biomedical applications, such as drug delivery systems and tissue engineering scaffolds.
- It can be used in the synthesis of various polymers with specific properties.
In practical use, divinyl sulfone’s reactivity needs to be carefully controlled, as its ability to crosslink can lead to the formation of highly crosslinked, insoluble polymers if not properly managed.
Divinyl sulfone acts as a cross-linking reagent for agarose gels. It is a monomer used in the production of polymers with diols, urea and malonic esters. Divinyl sulfone can be retained on a Primesep 100 mixed-mode column with great peak shape using an isocratic method of 1/99 Acetonitrile (ACN) and water. UV Detection 200 nm.
Condition
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN – 1.0% |
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | 200 nm |
| Peak Retention Time | 5.8 min |
| LOD* | 50 ppb |
| Sample (Divinyl Sulfone (stabilized with HQ)) | 0.2 mg/ml in H2O |
| Injection volume | 1 µl |
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed
Description
| Class of Compounds | Vinyl Monomer, Sulfone |
| Analyzing Compounds | Divinyl sulfone |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
