HPLC Determination of Fentanyl on Primesep 200 Column

HPLC Method for Fentanyl, Fentanyl citrate on Primesep 200 by SIELC Technologies

HPLC Determination of Fentanyl

High Performance Liquid Chromatography (HPLC) Method for Analysis of Fentanyl, Fentanyl citrate.

Fentanyl is an opioid drug with the chemical formula C22H28N2O.  It is a DEA Schedule II controlled substance due to it’s risk for potential abuse that could lead to a dependence. Fentanyl was developed in 1950 as an analgesic. It is often used to treat chronic cancer pain and surgical procedures. The compound does not appear in nature and is entirely laboratory made.

Fentanyl, Fentanyl citrate can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a [buffer] buffer. Detection is performed using UV.

Condition
Column Primesep 200, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 60/40%
Buffer H3PO4 – 0.1 %
Flow Rate 1.0 ml/min
Detection UV, 200 nm

 

Description
Class of compounds
 Narcotic, Drug, Basic, Hydrophobic, Ionizable, Zwitterionic
Analyzing Compounds Fentanyl, Fentanyl citrate

 

Application Column

Primesep 200

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Fentanyl
Fentanyl citrate
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.