HPLC Determination of Fosfomycin on Primesep SB Column

HPLC Determination of Fosfomycin
Fosfomycin is a broad-spectrum antibiotic consisting of an epoxide ring and a phosphite group. It can be retained on a Primesep SB column in about 8 minutes using an isocratic MS-compatible mobile phase method. Primesep SB is a reverse-phase (RP) mixed-mode column with embedded strong basic ion-pairing groups.


Column Primesep SB, 3.2x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 20/80%
Buffer AmFm pH 3.0- 20 mM
Flow Rate 0.5 ml/min
Detection CAD (Corona) MS- compatible mobile phase


Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Antibiotic
Analyzing Compounds Fosfomycin

Application Analytes: