HPLC Method for Determination of Gamma-aminobutyric acid (GABA), Vigabatrin, Phenibut, Pregabalin, Baclofen on Primesep 100 Column

Separation type: Liquid Chromatography Mixed-mode


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High Performance Liquid Chromatography (HPLC) Method for Analysis of Gamma-aminobutyric acid (GABA), Vigabatrin, Phenibut, Pregabalin, Baclofen

All gabapentinoids can be retained and separated on the Primesep 100 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and Ammonium Formate (AmFm) buffer. Detection can be achieved with mass spectrometry (MS), evaporative light scattering detection (ELSD) and Charged aerosol detection (CAD).

ColumnPrimesep 100,  4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN/H2O – 30/70%
BufferAmFm pH 3.0 – 50 mM
Flow Rate1.0  ml/min
DetectionCAD (Corona)  (MS-compatible mobile phase)
Class of Compounds
Acid, Drug
Analyzing CompoundsGamma-aminobutyric acid (GABA), Vigabatrin, Phenibut, Pregabalin, Baclofen

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Baclofen
Phenibut
Pregabalin
Vigabatrin
gamma-Aminobutyric Acid (GABA)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.