HPLC Determination of Glyphosate on Newcrom B Column

Separation type: Liquid Chromatography Mixed-mode





HPLC.cloud
View on hplc.cloud





High Performance Liquid Chromatography (HPLC) Method for Analysis of Glyphosate




Glyphosate is a broad-spectrum herbicide which is used to kill weeds. Presence of glyphosate is strongly regulated by various governing agencies in the US, Europe, and Asia. Glyphosate can be retained in HPLC with a Newcrom B mixed-mode column using a mobile phase of acetonitrile (ACN, MeCN), water and phosphoric acid (H3PO4) buffer allowing the use of a UV detector at 200 nm.




 

Condition
Column Newcrom B, 3.2×100 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer H3PO45 – 0.08%
Flow Rate 0.5 mL/min
Detection  UV 200 nm

Description
Class of Compounds Hydrophobic, Herbicide, Pesticide
Analyzing Compounds Glyphosate

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Glyphosate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.