HPLC Determination of Lysine on Primesep 200 column in MS-compatible conditions

Lysine is an essential amino acid used in the synthesis of proteins. In biological conditions, it is a basic, charged molecule. Lysine can be retained on Primesep 200 mixed-mode column which has embedded weak acidic ion-pairing groups for retention of basic analytes in HPLC. The mobile phase consisting of acetonitrile (ACN) and water with ammonium formate (AmFm) makes this method analysis MS-compatible.

Condition

Column Primesep 200, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 10/90%
Buffer AmFM pH 3.0 - 10 mM
Flow Rate 1.0 ml/min
Detection CAD (Corona), MS- compatible phase
 

Description

Class of Compounds Acid, Hydrophilic, Ionizable, Carboxylic acid, Drug, Amino Acid
Analyzing Compounds Lysine
 

Application Analytes:

D-Lysine
Lysine