HPLC Determination of Mycophenolate Mofetil on Primesep SB Column

HPLC Determination of Mycophenolate mofetil Mycophenolate mofetil alongside other medicines is used as an immunosuppressant to prevent organ rejection after a transplant. THe compound can be retained in HPLC using Primesep SB mixed-mode column that has both hydrophobic and anion-exchange properties. The analytical method’s mobile phase consists of a gradient of acetonitrile (ACN) in water with phosphoric acid (H3PO4) buffer and using UV detection at 250nm.


Column Primesep SB, 4.6x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-20%, 10 min
Buffer H3PO4 - 0.2 %
Flow Rate 1.0 ml/min
Detection UV, 250 nm


Class of Compounds Drug, Hydrophobic, Ionizable, Carbocyclic, Benzoate
Analyzing Compounds Mycophenolate Mofetil

Application Analytes:

Mycophenolate mofetil