HPLC Determination of Mycophenolic Acidon Primesep SB Column

HPLC Determination of Mycophenolic Acid Mycophenolic acid alongside other medicines is used as an immunosuppressant to prevent organ rejection after a transplant. THe compound can be retained in HPLC using Primesep SB mixed-mode column that has both hydrophobic and anion-exchange properties. The analytical method is isocratic and uses the mobile phase of acetonitrile (ACN) in water with phosphoric acid (H3PO4) buffer with UV detection at 226nm.  

Condition

Column Primesep SB, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 40/60%
Buffer H3PO4- 0.4%
Flow Rate 1.0 ml/min
Detection UV, 226 nm
 

Description

Class of Compounds Drug, Acid, Hydrophobic, Ionizable, Immunosuppressant
Analyzing Compounds Mycophenolic Acid
 

Application Analytes:

Mycophenolic acid
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