HPLC Determination of PEI complex with Cu(II)

HPLC Determination of PEI complex with Cu(II)_1177 Polyethyleneimine (PEI) is a polymer used in a wide range of applications. It is difficult to analyze in HPLC due to several factors. It’s a mixture of compounds with different lengths and therefore different number of charges. It also lacks a UV chromophore. New PEI column was designed specifically for the analysis of polyethyleneimine by ion-exclusion and size-exclusion mechanisms with copper complex to allow for UV detection. The method uses a mobile phase of acetonitrile (ACN) and water with ammonium formate buffer (AmFm) and UV detection at 285nm. MEASURING PEI Polyethylenimine in biological samples


Column PEI, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 40/60%
Buffer AmFM pH 3.0  - 20 mM
Flow Rate 0.5 ml/min
Detection UV, 285 nm


Class of Compounds Polymer, Basic, Hydrophilic, Ionizable
Analyzing Compounds PEI

Application Analytes:

Polyethylenimine PEI