HPLC Determination of PEI complex with Cu(II)

HPLC Determination of PEI complex with Cu(II)_1177
Polyethyleneimine (PEI) is a polymer used in a wide range of applications. It is difficult to analyze in HPLC due to several factors. It’s a mixture of compounds with different lengths and therefore different number of charges. It also lacks a UV chromophore. New PEI column was designed specifically for the analysis of polyethyleneimine by ion-exclusion and size-exclusion mechanisms with copper complex to allow for UV detection. The method uses a mobile phase of acetonitrile (ACN) and water with ammonium formate buffer (AmFm) and UV detection at 285nm.

MEASURING PEI Polyethylenimine in biological samples

Condition

Column PEI, 4.6×250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 40/60%
Buffer AmFM pH 3.0  – 20 mM
Flow Rate 0.5 ml/min
Detection UV, 285 nm

 

Description

Class of Compounds
Polymer, Basic, Hydrophilic, Ionizable
Analyzing Compounds PEI

 

Application Column

PEI

SIELC Technologies offers custom phases for customers who require specific separations not achievable with commercially available HPLC phases. Considering the vast array of compounds and mixtures requiring analysis, tailored LC phases can significantly enhance separation results for unique and challenging applications. To learn more about our special custom LC phases designed for your specific separation needs, please contact SIELC Technologies at research@sielc.com. Our team of experts is ready to guide you through the process and create a custom solution that addresses your particular chromatographic challenges.

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Application Analytes:
Polyethylenimine PEI
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.