HPLC Determination of Ractopamine on Primesep 100 Column

Ractopamine is a livestock feed additive designed to promote muscle synthesis and leanness. It is banned in many countries, excluding the US, Canada and Mexico. It can be retained on a Primesep 100 Column in about 7 minutes. Primesep 100 is a reverse-phase (RP) mixed-mode column with embedded acidic ion-pairing groups.


Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 20/80%
Buffer H2SO4 - 0.3 %
Flow Rate 1.0 ml/min
Detection UV, 222 nm


Class of Compounds Drug, Basic, Hydrophobic, Ionizable, Amine
Analyzing Compounds Ractopamine

Application Analytes:

Ractopamine hydrochloride